مقالات پذیرفته شده در پنجمین کنگره بین المللی زیست پزشکی
Considering the effect of fermented soybean meal by bacillus subtilis on CD177 gene expression in septic rat
Considering the effect of fermented soybean meal by bacillus subtilis on CD177 gene expression in septic rat
Fahimeh Ganjifar,1Azadeh Rasooli,2,*Reza Hojihosseini,3
1. Department of Biochemistry, Faculty of Science, Payame Noor University, Tehran, Iran 2. Department of Biochemistry, Faculty of Science, Payame Noor University, Tehran, Iran 3. Department of Biochemistry, Faculty of Science, Payame Noor University, Tehran, Iran
Introduction: Introduction
Sepsis as life-threatening acute organ dysfunction secondary to infection affects more people each year. After sepsis, the immune response is associated with a major inflammatory response. Neutrophils have various functions in the immune system in sepsis. CD177 as an important neutrophil gene encodes the glycoprotein of the membrane that the expression of this gene is increased during bacterial infections. So, the measurement of CD177 mRNA levels could be a useful diagnostic tool for distinguishing some diseases such as sepsis. Due to the high incidence of sepsis and high risk of death globally, this syndrome needs urgent treatment. Natural resources like soybean meal as an important source of protein are the richest bio-resource of drugs. Also, fermented soybean meal by mixed microorganism shows probiotic properties that can be as an alternative for antibiotics. Therefore, in this study, the anti-inflammatory effect of fermented soybean meal extract by bacillus subtilis on septic rat was investigated.
Methods: Methods
Sixty male Wistar rats (250±20 g) were purchased from the Pasteur Institute of Iran. Rats randomly divided into 6 groups (n=10) including Control groups (without any surgery and treatment), laparotomy groups (LAP) (Shock surgery groups without ligation and puncture of cecum), CLP groups (cecal ligation and puncture without treatment), and treatment groups (5%, 10% and 20% of fermented soybean meal after sepsis induction). The soybean meal was fermented with bacillus subtilis, and then its extract was prepared for further analysis (All procedures was done according Miri et al., (2021) article). After 48 h, the rats were euthanized and tissues were collected for Real time PCR analysis. Real-time PCR reactions were performed using SYBR Green PCR Master Mix (QIAGEN, Germany) with Rotor-Gene Q system (QIAGEN, Germany).
Results: Results
The results of this study revealed that the expression of CD177 gene as inflammatory factor increased by LAP and CLP surgery compared with control group in both lung and spleen tissues and animal treatments with fermented soybean in all doses have been effective in decreasing the expression level of the CD177 gene compared with the CLP group (P < 0.05). It is noteworthy that fermented soybean meal at a dose of 5% was better in diminishing of CD177 gene expression in both tissues as compared with the dose of 10 and 20 %.
Conclusion: Conclusion
Finally, the results showed that treating rats with fermented soybean meal immediately after sepsis induction reduced sepsis-induced lung and spleen tissues damage. Therefore, it can be concluded that anti-inflammatory effects of fermented soybean meal could be a potential therapeutic agent for tissues injury in sepsis induced by CLP.