• Histological survey on topical effect of Skin Pistachio hydroethanolic extract ointment on excisional wound healing in rat
  • Leila Zarei,1 Masoumeh Pourjabali,2 Asghar Rajabzadeh,3,*
    1. Associate Professor,Department of Anatomical Sciences, Faculty of Medicine, Lorestan University ofMedical Sciences, Khorramabad, Iran.
    2. Assistant Professor,Department of Pathology, Faculty of Medicine, Urmia University of MedicalSciences, Urmia, Iran.
    3. Assistant Professor,Department of Anatomical Sciences, Faculty of Medicine, Lorestan University ofMedical Sciences, Khorramabad. Iran.


  • Introduction: Wound healing is one of the most important issues that mankind faces from the beginning of creation. Therefore, trying to find compounds that affect the wound healing has the least side effects is one of the human aspirations and in this regard, medicinal plants are of particular importance. In this study, the effect of Skin pistachio hydroalcoholic extract ointment on the healing process was carried out based on planetary and histological studies in a 14-day period after the wound.
  • Methods: In order to evaluate the planter size, 20 healthy rats were randomly assigned into 5 groups of 4, including control group (open wound without any intervention and treatment), control group (open wound with ointment without extracts), treatment group 1 with concentration 1 % of ointment containing pistachio extract, the second group with 2% concentration of ointment containing skin pistachio extract and the treatment group 3 with 4% concentration of ointment containing pistachio extract were divided. The wounds are washed with normal saline solution for 10 days (daily) and will be applied to ointment groups.
  • Results: The results showed that wound treatment with this ointment in the experimental group resulted in a significant increase (p <0.05) in the percentage of wound healing. Also, there was a significant difference in the thickness of skin and collagen diameter and reduction of edema (P <0.05) was observed. Comparison of the results of neutrophil count, fibroblast, and macrophage was observed on day 14 in the treated groups with skin pistachio extract and control (p <0.05). These results were indicative of accelerated wound healing in treated specimens.
  • Conclusion: According to the results of this study, an ointment containing skin pistachio extract can have healing effects on the wound created in the skin of the rat.
  • Keywords: Full thickness wound, Wound healing, hydroalcoholic extract, skin Pistachio, Histology, planimetric