• Association study Between rs7975232 Polymorphism in Vitamin D Receptor Gene and Periodontitis by Tetra Arms PCR
  • Nafiseh Tavakoli,1 Elham Siasi,2,* Kumars Amini,3
    1. Master of Genetics, Department of Genetic, Faculty of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
    2. Master of Genetics, Department of Genetic, Faculty of Biology Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
    3. Associate Professor, Department of Microbiology, Faculty of Biology Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran


  • Introduction: Periodontitis is an inflammatory multifactorial disease in tooth tissues. Many genetic reasons and environmental factors are caused this disease. Vitamin D deficiency, is determined related to periodontitis disease risk. In this study was investigated of the association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis in Iranian patient and control groups.
  • Methods: Blood samples from 100 samples (50 patients and 50 controls group) were selected and DNA from samples was extracted by DNA extraction kit. Genotyping was used by Tetra Arms PCR method. Tetra Arms PCR genotyping results was confirmed by sequencing. Then results were statistical analyzed by SPSS (Statistical Package for the Social Sciences) software.
  • Results: Frequency of AA, AC and CC Genotypes were 25 (50%), 14 (28%), and 11 (22%) in cases, and 26 (52%), 16 (32%) and 8 (16%) in controls, respectively. AA genotype was the highest genotype between patient and control groups. Statistical analysis showed no significant association between this polymorphism and periodontitis disease in the studied samples (p=0.67).
  • Conclusion: This finding showed there was not significant association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis disease in the Iranian studied samples. For confirming of this results, further studies with large sample size and different types of population, was recommended.
  • Keywords: Periodontitis disease, Single nucleotide polymorphism, Vitamin D receptor, Tetra arms-PCR.