• The effect of caffeic acid phenethyl ester on Interleukin 10 (IL-10) and Interleukin 33 (IL-33) anti-inflammatory cytokine expression in Wistar rat model with Alzheimer's disease
  • Fatameh Rouhollah,1,* Maryam saeidi,2 Shadi Jafari,3 Esmaeil Emdadi Siyah Pirani,4 Seyed Hamid Jamaloddini,5
    1. Department of Molecular and Cellular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    2. Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    3. Department of Molecular and Cellular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
    4. Department of Molecular and Cellular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    5. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran


  • Introduction: Alzheimer disease is one of the brain functional disorder, which influenced on memorize and mental ability of patients, slowly. Interleukin-10 acts as an immune regulator in inflammatory situation. Also Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The Caffeic acid (3, 4- dihydroxy cinnamic acid) is one of the most importance of hydroxycinnamic acid in Propolis that its antioxidant effect could block NF-kappa B pathway
  • Methods: The present study was performed on wistar rat, these animals were divided into 6 groups (control, sham, scopolamine, 1, 3 and 8 Caffeic acid phenethyl ester recipients). The DNA was extracted from their brain tissue. Then IL-10 and IL 33 genes expression was evaluated by QRT-PCR. The histopathological examinations were performed on brain sections
  • Results: The number of remaining healthy neurons in the Caffeic acid phenethyl ester treated group 3 mg was more than the number of these cells in the treatment group with Caffeic acid phenethyl ester 1 mg. Although the highest number of normal cells were observed in sham group and treated with Caffeic acid phenethyl ester 8 mg. The molecular studies also showed that the expression level of IL-10 and IL-33 genes were significantly increased in the group treated with 3 mg drug.
  • Conclusion: Using an effective dose of Caffeic acid phenethyl ester, as an anti-inflammatory compound, can improve Alzheimer through impact on the IL-10 and IL-33 genes expression and the memory and learning improvement and reducing cellular damage. However, more extensive studies are still needed in this area
  • Keywords: Alzheimer, Caffeic acid phenethyl ester, Interleukin 10, Interleukin 33, wistar rat