• The effect of Injectable platelet rich fibrin (i-PRF) on inflammation in the mouse ovarian tissue after autotransplantation
  • Sahar Hatami,1,* Seyed Mohammadali Shariatzadeh,2 Malek Soleimani Mehranjani,3
    1. Phd student, Department of Biology, Faculty of Science, Arak University
    2. Department of Biology, Faculty of Science, Arak University
    3. Department of Biology, Faculty of Science, Arak University


  • Introduction: Ovariaan tissue transplantation is only option for patiant young woman and prepubertal girls after chemotherapy and radiotherapy. Howere one of the major limitations in ovary transplantation is ischemia-reperfusion (I/R) injury that due to the production of inflammatory factors and finelly, reduction survival of transplanted tissue. Injectable platelet rich fibrin (i-PRF) is a liquid formulation of platelet rich fibrin (PRF) without the use of anti-coagulants. It contains a fibrin matrix resulted from fibrinogen molecules activation in plasma, leukocytes, circulating stem cells, platelets and growth factors. We aimed to evaluate the effect of i-PRF bioscaffold on the serum level of inflammatory factors such as interlukin 6, 10 (IL6,10) and Tumor necrosis factor-α (TNF-α) following mouse ovarian tissue transplantation.
  • Methods: Mice were divided into (n=6): control, autograft + saline (whole ovarian tissue transplanted in the gluteus superficialis muscle, saline directly injected into it), autograft + i-PRF (whole ovarian tissue transplanted in the gluteus superficialis muscle, i-PRF was directly injected into it ). 7 days after ovary transplantation, serum concentrations of IL-6, IL-10 and TNF-α were assayed. Data were analyzed using one-way ANOVA and Tuckey’s test and the means were considered significantly different at p-value < 0.05.
  • Results: Serum concentrations of TNF-α and IL-6 in the autograft group increased significantly compared to the control, while it showed a significant reduction in the autograft + i-PRF group compared to the autograft group. Moreover, the serum level of IL-10 was significantly lower in the autograft group when compared to the control counterpart. Whereas it showed a significant increase in the autograft + i-PRF group compared to the autograft group (p < 0.05).
  • Conclusion: Our results for the first time revealed that i-PRF bioscaffold at the graft site can decrease inflammation. therefore can prevent IR induced damages and improve the function of the grafted ovary.
  • Keywords: Ovarian tissue transplantation, Injectable platelet rich fibrin (i-PRF), Ischemia–reperfusion, Infla