مقالات پذیرفته شده در پنجمین کنگره بین المللی زیست پزشکی
Injectable platelet rich fibrin (i-PRF) bioscaffold improves total antioxidant capacity in mouse transplanted ovarian tissue
Injectable platelet rich fibrin (i-PRF) bioscaffold improves total antioxidant capacity in mouse transplanted ovarian tissue
Sahar Hatami,1,*Seyed Mohammadali Shariatzadeh,2Malek Soleimani Mehranjani,3
1. Phd student, Department of Biology, Faculty of Science, Arak University 2. Department of Biology, Faculty of Science, Arak University 3. Department of Biology, Faculty of Science, Arak University
Introduction: However, Ovarian tissue transplantation can be a promising solution for preserve fertility return in cancerous women and girls. But, ischemia-reperfusion (I/R) injury occurs during the early post-transplantation days leads to oxidative stress and reduction graft function and long-term survival of the transplanted organ. Injectable platelet rich fibrin (i-PRF) is a liquid formulation of platelet rich fibrin (PRF) without the use of anti-coagulants. i-PRF is bioscaffold that contains leukocytes, circulating stem cells, platelets and growth factors. In the present study, we investigated the effect of i-PRF bioscaffold on the serum level of malondialdehyde (MDA) and total antioxidant capacity (TAC) after mouse ovarian tissue transplantation.
Methods: 18 Mice were randomly divided into three groups): control, autograft + saline (whole ovarian tissue transplanted in the gluteus superficialis muscle, saline directly injected into it), autograft + i-PRF (whole ovarian tissue transplanted in the gluteus superficialis muscle, i-PRF was directly injected into it ). Serum concentrations of MDA and TAC were measured 7 days after ovary transplantation. Data were analyzed using one-way ANOVA and Tuckey’s test and the means were considered significantly different at p-value < 0.05.
Results: In the autograft group, Serum concentrations MDA significantly increased in compare to the control, while it showed a significant decrease in the autograft + i-PRF group compared to the autograft group (p < 0.05). Moreover, the serum level of TAC decreased significantly in the autograft group compared to the control counterpart, whereas it increased significantly in the autograft + i-PRF group compared to the autograft group (p < 0.05).
Conclusion: These data showed that i-PRF bioscaffold at the graft site can decrease stress oxidative. therefore can prevent IRI injury through increasing total antioxidant capacity.