Improvement of the proliferation and stemness of human hair follicle stem cells via low level laser irradiation

Improvement of the proliferation and stemness of human hair follicle stem cells via low level laser irradiation
Mina Sadat Naderi,¹ Seyed Mehdi Tabaie,^2,*
1. Assistance Professor, PhD of Biophysics, Department of Biophysics, Faculty of Biological Sciences, Islamic Azad University, Tehran North Branch ,Tehran, IRAN
2. MD, Dermatologist, Associated Professor, Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, IRAN
Introduction: Low level lasers are currently used for many medical applications, including wound healing as well as hair loss treatment. Hair follicle stem cells (HFSCs) are used as a model for the primary skin papilla cell system. HFSCs are attractive candidates for clinical repair and regenerative medicine. The methods that augment the stemness characteristics improved applications of stem cells. Low level laser radiation can stimulate cell proliferation. In some studies, the effect of low level laser has been investigated for the treatment of androgenic alopecia. It should be noted that low level laser can increase cell proliferation by affecting the NF-κB pathway.
Methods: In this study, the effect of low level laser irradiation on the proliferation and stemness gene of human hair follicle stem cells were investigated. At first, hair follicle stem cells were extracted by enzymatic and mechanical methods, and then confirmation tests of hair follicle stem cells were performed by flow cytometry. After the laser power meter, the potential of the low level laser with the energy density of 1 and 5 J/cm2 as a treatment on the proliferation and also expression of stemness genes such as Sox2, Oct4 and Slug was investigated.
Results: The result of this study indicate that the low level laser irradiation increase the proliferation of HFSCs under the 5 J/cm2 irradiation. Expression of stemness genes (Sox2, Oct4 and Slug) also increase. low level laser irradiation not only can be preventing the decrement of the stemness characterized but also increase the expression of stemness genes of the hair follicle stem cells.
Conclusion: This result paves the way for utilizing the hair follicle stem cells for effective applications in cell therapy and wound healing.
Keywords: hair follicle Stem cells, Low level laser, Stemness