مقالات پذیرفته شده در پنجمین کنگره بین المللی زیست پزشکی
Genetic assessments in dentigerous cyst
Genetic assessments in dentigerous cyst
Shirin Saravani,1,*Hamideh Kadeh,2
1. Oral & Dental Disease Research Center, Department of Oral & Maxillofacial Pathology, Zahedan University of Medical Science, Zahedan, Iran 2. Oral & Dental Disease Research Center, Department of Oral & Maxillofacial Pathology, Zahedan University of Medical Science, Zahedan, Iran
Introduction: Dentigerous cyst (DC) is the most common odontogenic cyst, which results from the accumulation of fluid between the reduced enamel epithelium and the crown of the tooth. The role of genetic changes in the pathogenesis of this cyst has not been precisely elucidated. The P53 mutation was seen in codon 237 and the FHIT LOH mutation was seen in 10% of dentigerous cysts. In addition, mutation of PTCH gene has been identified as Loss of heterozygosity for D95287 and D95180 markers in 50% of cases of dentigrous cysts. LOXL4 mRNA expression was also seen in dentigerous cysts but was 3.7 times lower than in odontogenic keratocysts. 1qht chromosome polymorphism has also been observed in dentigerous cysts. Evaluation of genetic aberrant in dentigerous cysts has been done in few studies and most studies have examined the gene expression of different proteins by immunohistochemistry. These studies showed immunohistochemical expression of sonic hedgehog signaling pathway markers, Ki-67, CD138, RANK/RANKL, OPG, clusterin, P63, PCNA, elafin, Extracellular Matrix Metaloproteinase inducer in dentigerous cysts. In this study, we try to review the research done on genetic aberrant and expression of different proteins on dentigerous cysts.
Methods: Dentigerous cyst (DC) is the most common odontogenic cyst, which results from the accumulation of fluid between the reduced enamel epithelium and the crown of the tooth. The role of genetic changes in the pathogenesis of this cyst has not been precisely elucidated. The P53 mutation was seen in codon 237 and the FHIT LOH mutation was seen in 10% of dentigerous cysts. In addition, mutation of PTCH gene has been identified as Loss of heterozygosity for D95287 and D95180 markers in 50% of cases of dentigrous cysts. LOXL4 mRNA expression was also seen in dentigerous cysts but was 3.7 times lower than in odontogenic keratocysts. 1qht chromosome polymorphism has also been observed in dentigerous cysts. Evaluation of genetic aberrant in dentigerous cysts has been done in few studies and most studies have examined the gene expression of different proteins by immunohistochemistry. These studies showed immunohistochemical expression of sonic hedgehog signaling pathway markers, Ki-67, CD138, RANK/RANKL, OPG, clusterin, P63, PCNA, elafin, Extracellular Matrix Metaloproteinase inducer in dentigerous cysts. In this study, we try to review the research done on genetic aberrant and expression of different proteins on dentigerous cysts.
Results: Dentigerous cyst (DC) is the most common odontogenic cyst, which results from the accumulation of fluid between the reduced enamel epithelium and the crown of the tooth. The role of genetic changes in the pathogenesis of this cyst has not been precisely elucidated. The P53 mutation was seen in codon 237 and the FHIT LOH mutation was seen in 10% of dentigerous cysts. In addition, mutation of PTCH gene has been identified as Loss of heterozygosity for D95287 and D95180 markers in 50% of cases of dentigrous cysts. LOXL4 mRNA expression was also seen in dentigerous cysts but was 3.7 times lower than in odontogenic keratocysts. 1qht chromosome polymorphism has also been observed in dentigerous cysts. Evaluation of genetic aberrant in dentigerous cysts has been done in few studies and most studies have examined the gene expression of different proteins by immunohistochemistry. These studies showed immunohistochemical expression of sonic hedgehog signaling pathway markers, Ki-67, CD138, RANK/RANKL, OPG, clusterin, P63, PCNA, elafin, Extracellular Matrix Metaloproteinase inducer in dentigerous cysts. In this study, we try to review the research done on genetic aberrant and expression of different proteins on dentigerous cysts.
Conclusion: Dentigerous cyst (DC) is the most common odontogenic cyst, which results from the accumulation of fluid between the reduced enamel epithelium and the crown of the tooth. The role of genetic changes in the pathogenesis of this cyst has not been precisely elucidated. The P53 mutation was seen in codon 237 and the FHIT LOH mutation was seen in 10% of dentigerous cysts. In addition, mutation of PTCH gene has been identified as Loss of heterozygosity for D95287 and D95180 markers in 50% of cases of dentigrous cysts. LOXL4 mRNA expression was also seen in dentigerous cysts but was 3.7 times lower than in odontogenic keratocysts. 1qht chromosome polymorphism has also been observed in dentigerous cysts. Evaluation of genetic aberrant in dentigerous cysts has been done in few studies and most studies have examined the gene expression of different proteins by immunohistochemistry. These studies showed immunohistochemical expression of sonic hedgehog signaling pathway markers, Ki-67, CD138, RANK/RANKL, OPG, clusterin, P63, PCNA, elafin, Extracellular Matrix Metaloproteinase inducer in dentigerous cysts. In this study, we try to review the research done on genetic aberrant and expression of different proteins on dentigerous cysts.