• Investigating of IL12B gene expression in blood cells of patients with Secondary Progressive MS in zanjan
  • Katayoon Rahmani,1 Sanaz Mahmazi,2,* Mohsen Kalantari,3
    1. Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
    2. Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
    3. Assistant professor of Molecular Genetics, Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.


  • Introduction: Multiple sclerosis (MS) is one of the most common neurological diseases in adolescents. MS is usually seen in people between the ages of 15-50. MS is about three times more common in women than in men, and the disease can occur at any stage of life. The symptoms of Secondary progressive MS always begin as RRMS, but the clinical symptoms change in such a way that the patient experiences steady severe symptoms without being associated with acute attacks. The risk of infection in this type of MS is 2.5%. The main purpose of this study was to evaluate the expression of the IL-12B gene in the blood cells of patients with MS compared to normal samples.
  • Methods: In this study, blood samples were collected from healthy individuals for the control group as well as venous blood samples from patients with MS from the neurology of Valiasr Hospital in Zanjan. Written consents were obtained from all individuals in both patients and the control group. Then RNA extraction and cDNA synthesis were performed and the expression changes of IL12B and Bact as the Housekeeping gene were evaluated by Real-Time PCR. Finally, the results of Real-Time PCR were analyzed by Rest 2002 Software and related diagrams were drawn by Excel
  • Results: IL-12 gene expression in patients with SPMS increased 7.3 times compared with the control group. This change in expression was statistically significant and Bact gene was the reference gene.
  • Conclusion: The results of relative analysis of IL-12 gene expression in patients with SPMS compared to the control group showed that gene expression in these patients increased 7.3 times compared to the normal group. This change in expression was statistically significant. Data analysis was performed for all samples separately and in comparison, to the control group, most of the samples were statistically significantly different (P <0.05). IL-12 gene is one of the most important biomarkers in MS, and the results of our study confirmed the increase of this gene in SPMS.
  • Keywords: IL12B, Bact, Housekeeping, cDNA