• Evaluation of application zinc nanoparticles with chitosan biofilm on healing of infected wound with methicillin-resistant Staphlococcus aureus (MRSA) in rat
  • Leila Zarei,1 Asghar Rajabzadeh,2,*
    1. .Associate Professor,Department of Anatomical Sciences, Faculty of Medicine, Lorestan University ofMedical Sciences, Khorramabad, Iran.
    2. Assistant Professor,Department of Anatomical Sciences, Faculty of Medicine, Lorestan University ofMedical Sciences, Khorramabad. Iran.


  • Introduction: The present study was aimed at assessment of effect of application of zinc nanoparticles with chitosan biofilm on healing of infected wound with Methicillin- resistant Staphylococcus aureus (MRSA) in rat.
  • Methods: Sixty-eight male Wistar rats were randomized into four groups of 17 animals each. In group I (Normal) the wounds were created with no infection. In group II (CHIT), the wounds with no infection were dressed by chitosan biofilm. In group III (MRSA/CHIT), animals with infected wounds were dressed with chitosan biofilm only. In group IV (MRSA/CHIT/NZ), animals with infected wounds were dressed with Chitosan/Nano zinc biofilm.
  • Results: There were significant differences in comparisons of group IV and other groups, particularly in terms of cellular infiltration and neovascularization. During the study period, scores for neovascularization was significantly higher in group IV rats than other groups (P <0.05). Polymorphonuclear (PMN) and mononuclear (MNC) cell count and fibroblast cell proliferation in group IV were significantly higher than those of other experimental groups (P <0.05).
  • Conclusion: Chitosan/Nano zinc biofilm resulted in significant improvement in histopathological indices in full thickness infected wound healing.
  • Keywords: Infected wound, Chitosan/Nanozinc, biofilm, rat