The effect of resveratrol in comparison with cisplatin on cytotoxicity and production of reactive oxygen species in a prostate cancer cell line (LNCap)
The effect of resveratrol in comparison with cisplatin on cytotoxicity and production of reactive oxygen species in a prostate cancer cell line (LNCap)
Nasim kojoeiyan Jafari ,1,*Mohammad Taghi Goodarzi,2
1. Department of Biochemistry, Islamic Azad University, Shahrood Branch, Shahrood, Iran 2. Department of Biochemistry, Islamic Azad University, Shahrood Branch, Shahrood, Iran
Introduction: Objective Prostate cancer is one of the most common diseases worldwide, and many efforts are made to treat that. However, no absolute treatment is recognized for the disease. Accordingly, this study tends to investigate the effects of resveratrol in comparison with cisplatin on the cytotoxicity and production of reactive oxygen species (ROS) in a prostate cancer cell line (LNCap).
Methods: Methods After treatment of LNCap cells with concentrations of 1, 10, 100, and 1000µg/ml of resveratrol, and 1, 5, 10, and 50µg/ml of cisplatin, the viability percentage of cells was analyzed using MTT and spectrophotometry methods. Also, the reactive oxygen species (ROS) production in this cell line with a concentration of IC50 cisplatin and resveratrol was analyzed using the flow cytometry (FC) method.
Results: Results The results of MTT test showed that cisplatin and resveratrol can both reduce the cell viability in high concentrations because of increased cytotoxicity. Measurement of ROS production level showed that cisplatin and resveratrol increased ROS levels compared to control cells.
Conclusion: Conclusion The results obtained from this study showed that resveratrol as a plant derivative has anticancer and antioxidant properties. According to the side effects of cisplatin, it can be replaced by resveratrol as a good candidate to be used in chemotherapy.
Keywords: Keywords: prostate cancer, resveratrol, cisplatin, reactive oxygen species (ROS)