nanofibrous mat loaded with growth factors, silver sulfadiazine and hair follicle bulge stem cells can facilate wound healing in rat
nanofibrous mat loaded with growth factors, silver sulfadiazine and hair follicle bulge stem cells can facilate wound healing in rat
fereshteh nejaddehbashi,1,*Eskandar moghimipour,2mahmoud orazizadeh,3vahid bayati,4mohammadreza abbaspour,5Mahmoud hashemitabar,6
1. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2. Nanotechnology Research center, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 3. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute,Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 4. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute,Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 5. Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran 6. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute,Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Introduction: Background: Despite special advances in regenerative medicine, wound healing is a challenging medical problem. The skin is the largest organ in the human body that has essential and important functions, so any damage to its normal structure should be treated as soon as possible. Easy access to skin stem cells has received a great deal of attention in its therapeutic applications. Cell therapy is a new method in restorative medicine, especially when old therapies have disappeared. Candidate populations for therapeutic applications include mesenchymal stem cells, hair follicle stem cells, and pluripotent cells. Hair follicle bulge stem cells with scaffold and growth factors can improve wound healing.
Methods: Materials and methods: In this study PCL/SSD, PCL/Coll as two -layered nanofibrous mat were designed with electrospinning. Bulge stem cells were isolated from rat vibrissa and cultured on two – layered nanofibrous mat with nanoparticles containing EGF and bFGF as growth factors. Nanoparticles containing EGF and bFGF were made with ionic gelation processes. EGF and bFGF release were analyzed with human EGF and bFGF Quntikine human ELIZA kit. Bulge stem cells were analyzed with Immunocytochemistry and flow cytometry. These cells were tracked with CM-Dill 7000C. Tracked cells were seeded on nanofibrous mat and grafted to rat skin. The healing process was evaluated with macroscopic observation on 7,14,21 and 28 days' post wounding. Hematoxylin - eosin and Trichromasons staining was done to evaluate tissue remodeling and collagen synthesis. Real time PCR analysis performed to assess gene expression after 14 days of treatment.
Results: Results: This combination of nanofibrous mat with nanoparticles containing growth factors and rat hair bulge stem cells showed normal characteristics with desirable skin wound healing. Moreover, attachment and spreading of bulge stem cells on this nanofibrous composite was reported to be in an optimal condition. As well, in vivo evaluation demonstrated that full repaired skin had been observed on day 14 in the two-layered nanofibrous mat with bulge stem cells and nanoparticles containing growth factors group after being wounded.
Conclusion: Conclusion: The two-layered nanofibrous mat with bulge stem cells and nanoparticles containing growth factors could be considered as a suitable wound dressing of nanofibrous membranes in future in order to shorten healing time.