Title: Leukoreduction Filters; a New Source of Microparticle for Human and Animal Study
Title: Leukoreduction Filters; a New Source of Microparticle for Human and Animal Study
Mohammad Reza Javan,1,*Sahar balagholi,2Sedigheh Amini Kafi-Abad,3samira karami,4Rasol dabbaghi,5
1. Blood Transfusion Research Center, High Institute for Research and Education in transfusion Medicine, Tehran, Iran. 2. Blood Transfusion Research Center, High Institute for Research and Education in transfusion Medicine, Tehran, Iran. 3. Blood Transfusion Research Center, High Institute for Research and Education in transfusion Medicine, Tehran, Iran. 4. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 5. Department of Hematology, Faculty of Medical sciences, Tarbiat Modares University, Tehran, Iran
Introduction: The isolation of microparticle from leukoreduction filters (LRFs) during cell extraction process can be introduced LRFs as precious source of Microparticle for animal and human study.
Methods: 10BioR blood filters were collected from Tehran Blood Transfusion Center. Back-flushing method was used for microparticle isolation from the LRFs. Dynamic light scattering (DLS), electron microscopy (EM), and flowcytomerty were performed for evaluation of microparticle size, Morphology, and structural properties respectively. Statistical analyses were carried out for evaluation of different between test and control groups. a p value of less than 0.05 indicates that a difference is significant.
Results: DLS analysis showed that the average microparticle size in the test and control samples was 235.7 nm and 189.5 nm respectively. We were able to separate 1202095.34 and 280948.64 microparticles from each test and control samples, respectively and the difference was significant (p value<0.008). We proved that the count of CD41, CD14, CD34 and CD235a microparticles in tests sample were 348213, 271378.4, 21464.6 and 57864.9 respectively while in control samples CD41, CD14, CD34 and CD235a microparticle count were 59246.2, 24167.6, 3033.2 and 96526.8 respectively. Statistical analysis showed the significant difference between the count of only CD14 and CD41 microparticles between test and control samples (p value<0.008). SEM showed that spherical, oval, cell fragment and micro-aggregate shapes while TEM revealed mitochondrial body in the samples.
Conclusion: It is concluded that LRFs, which until now were considered as blood transfusion waste, can be a source for extracting a large volume of cells microparticles with different structural properties for animal and human phase studies.