• Study of PHOX2B , ELP1, and MAP2 genes expression in bone marrow-derived mesenchymal stem cells after treatment by glabridin for differentiation into nerve-like cell
  • Maryam Hamidi,1 Hossein Fahimi,2 Hora Jalali Tehrani,3,*
    1. Department of Developmental Biology, Faculty of Advanced Science and Technology, Tehran Medical sciences , Islamic Azad university ,Tehran, Iran
    2. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical sciences , Islamic Azad university ,Tehran, Iran
    3. Department of Developmental Biology, Faculty of Advanced Science and Technology, Tehran Medical sciences , Islamic Azad university ,Tehran, Iran


  • Introduction: Mesenchymal stem cells can differentiate into all types of cells. Mesenchymal stem cells derived from bone marrow can differentiate into neuron- like cells in vitro conditions. Important genes in this process are ELP1, PHOX2B and MAP2. Glabridin is a polyphenolic flavonoid derived from the roots of licorice. Use of glabridin on cell differentiation was studied.
  • Methods: Mesenchymal stem cells derived from bone marrow was incubated. Toxication levels of glabridin was determined by MTT Assey test in concentrations of 5, 10, 20, 40 and 80 µM. CDNA was synthesized and the amount of change in gene expression was studied by real time PCR.
  • Results: Glabridin fatality dosage was tested by MTT in 40 and 80 µM concentration and based on 24 hour analysis, ELP1, PHOX2B and MAP2 gene expression in samples incubated with glabridin had significant increase compared to the control group.
  • Conclusion: Results from the study showed that glabridin of mesenchymal stem cells derived from bone marrow cause increased ELP1, PHOX2B and MAP2 gene expression resulting in increase in their differentiation into neuron- like cells. So glabridin can be used for increased differentiation into neuron- like cells.
  • Keywords: Mesenchymal stem cells, Neuron- like cell, Glabridin