The study of XBP1s factor as an indicate of ER stress pathway in tumor tissue of patient with colorectal cancer in compared to their margins from 1396 to 1398 in Imam Khomeini hospital
The study of XBP1s factor as an indicate of ER stress pathway in tumor tissue of patient with colorectal cancer in compared to their margins from 1396 to 1398 in Imam Khomeini hospital
Mohammadkian zarafshani,1Marveh rahmati,2,*Habibolah Mahmoodzadeh,3Vahid Soleimani,4Ahad Mohammadnejad,5
1. Tehran University of Medical Sciences School of Medicine, Department of General Surgery ,Cancer Institute of Iran , Cancer Biology Research Center. 2. Tehran University of Medical Sciences School of Medicine ,Cancer Institute of Iran , Cancer Biology Research Center. 3. Tehran University of Medical Sciences School of Medicine, Department of General Surgery ,Cancer Institute of Iran 4. Assistant Professor of Pathology Department of Pathology, School of Medicine Cancer Institute Tehran University of Medical Sciences 5. Tehran University of Medical Sciences School of Medicine ,Cancer Institute of Iran , Cancer Biology Research Center.
Introduction: Colorectal cancer (CRC) is one of the most common cancers with increasing mortality in Iran and the world. Despite new treatments relapse, drug resistance, and poor prognosis are some of the major challenges in patients with CRC. Recently, endoplasmic reticulum stress pathway (ER STRESS), especially XBP1s, has been emphasized as an important factor in the pathogenesis and targeted treatment of many types of cancer. Therefore, the aim of this project was to evaluate the expression of XBP1s as an indicator of the stress pathway of the ER stress in tumor tissues of patients with CRC compared to their tumor margins.
Methods: For this purpose, 91 Formalin-Fixed Paraffin-Embedded tissue sections of patients with CRC and 6 blocks of tumor margins as normal tissue were collected from tumor bank of Imam Khomeini Hospital in Tehran. XBP1s expression was measured by tissue microarray. Data analysis was performed using SPSS20 software with Chi-squared, Fisher exact and Kruskal-wallis test. In all experiments, p≤0.05 was considered statistically significant.
Results: Statistical analysis showed that there is a significant relationship between the expression of XBP1s in tumor tissues with the variable TNM (CLINICAL STAGE) and the degree of differentiation, (P value <0.05).
Conclusion: In conclusion ER stress pathway can be introduced as an indicator of diagnostic and therapeutic potential in colorectal cancer.
Keywords: Colorectal Cancer (CRC), Endoplasmic reticulum stress, tissue microarray, XBP1s