• Evaluation of genetic pattern of the most common cause of recurrence of bacterial urinary tract infection in kidney transplant patients, Tehran, Iran
  • Maryam Keshtvarz,1,* Mohammad Reza Pourmand,2 Zahra Zareh,3 Mohammad Ali Haghighi,4
    1. Bushehr University Of Medical Sciences, Bushehr, Iran
    2. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences
    3. Bushehr University Of Medical Sciences, Bushehr, Iran
    4. Bushehr University Of Medical Sciences, Bushehr, Iran


  • Introduction: Renal transplantation is the choice treatment for patients at end stage renal disease. Urinary tract infection (UTI) is one of the most common complications after renal transplantation, and it has serious consequences. Plused- Field Gel Electrophorsis (PFGE) is a new and accurate method for epidemiological studies of pathogenic organisms. The aim of this study was assessing UTI and recurrence in renal transplanted patients and used the PFGE method to obtain the genetic pattern of the most common cause of urinary tract infection and recurrence .
  • Methods: In this study, bacterial urinary tract infections in renal transplant recipients were determined.. The urine samples were taken from 94 hospitalized patients within 8-10 days after catheter removal and re-samples during the 6 months after transplantation for detection of recurrence , urinalysis and colony count were performed. Susceptibility of all the isolates to different antibiotics was determined by agar disk diffusion method and then the genetic patterns of the commonest isolated bacteria were obtained by PFGE and compared with patterns of antibiotic resistance.
  • Results: UTI was observed in 29 of patients and the most prevalent microorganism was E. coli 14(14.7%). Recurrence UTI occurred in 15(15.8%) patients and the main agent pathogen involved in recurrence was E.coli. Most of the isolated bacteria were susceptible to imipenem and resistant to Cotrimoxazole and Piperacillin. 13 patterns of antibiotic resistance and 13 DNA profiles in hospitalized patients and 14 patterns of antibiotic and 12 DNA profiles in outpatients were recognized.
  • Conclusion: Our study confirmed that bacterial infections remain as the most common infectious complication in the early post transplant period, and antibiogram rather than empirical treatment is needed to find the best effective antibiotics. With regard to high differentiation power of PFGE method, obtained patterns and high diversity of these profiles no epidemic UPEC was determined in hospitalized patients and outpatients. So, our result suggest that the most of recurrence UTIs in renal transplant patients caused by the same isolates.
  • Keywords: Urinary Tract Infections, Renal transplantation, E. coli, PFGE