• USING CURCUMIN TO PREVENT STRUCTURAL AND BEHAVIORAL CHANGES OF MEDIAL PREFRONTAL CORTEX INDUCED BY SLEEP DEPRIVATION IN RATS
  • Fatemeh Karimi,1,* Ali Noorafshan,2 Saied Karbalay-Doust,3 Ali Mohammad Kamali,4
    1. Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
    2. Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
    3. Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
    4. Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran


  • Introduction: Sleep Deprivation (SD) is known to result in a range of neurological consequences in chronically-afflicted subjects. Curcumin, a natural substance, has neuroprotective properties. This study aimed to evaluate the effects of curcumin on the medial Prefrontal Cortex (mPFC) of SD rats
  • Methods: Male rats were arbitrarily assigned to nine groups, including control, curcumin (100 mg/kg/day), olive oil, SD, SD+curcumin, SD+olive oil, grid, grid+curcumin, and grid+olive oil groups. SD was induced by a multiplatform box containing water. After a period of 21 days, the learning and memory of the rats were tested in an eight-arm radial maze. Afterwards, their brains were evaluated using stereological methods
  • Results: Concomitant treatment of curcumin during SD caused fewer errors during evaluation of the working and reference memory errors in the acquisition and retention phases. The overall volume of the mPFC, Infralimbic Cortex (ILC), Prelimbic Cortex (PLC), Anterior Cingulate Cortex (ACC) and the total number of neurons and glial cells reduced by 20 %-40 % on average in the SD animals in comparison to the control group. This indicated atrophic changes and cell loss in these areas (p < 0.01). The dendrites’ length and the number of spines per dendrite also reduced by 35 %-55 % in the SD rats compared to the ones in the control group (p < 0.01). Yet, treatment of the SD animals with curcumin prevented the atrophic changes of the mPFC, cell loss, and dendritic changes (p < 0.05)
  • Conclusion: SD induced structural changes in the mPFC and memory impairment in the rats. However, curcumin could protect their PFC
  • Keywords: Sleep deprivation, mPFC, curcumin, stereology, rat