Protective Effect of Nano Emulsions Containing Rosemary on Hippocampal CA1 Pyramidal Neurons in a Rat model of Cerebral Ischemia-Reperfusion
Protective Effect of Nano Emulsions Containing Rosemary on Hippocampal CA1 Pyramidal Neurons in a Rat model of Cerebral Ischemia-Reperfusion
Zahra Abbasy,1,*
1. Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
Introduction: Stroke is an important cause of mortality and morbidity worldwide but effective therapeutic strategy for the prevention of brain injury in patients with cerebral ischemia is lacking. Rosemary is a plant that have strong antioxidant and anti-inflammatory effects that may be helpful.
Methods: This study had two main parts: In vivo and in vitro. In in vivo part, we divided wistar rats into 8 groups (control, ischemia/reperfusion, 3 dose of aqueous- alcoholic extracts of rosemary and 3 dose of aqueous extracts of rosemary), after 21 days of rosemary administration the ischemia and reperfusion was done, finally apoptosis gene and neurons death were assayed in hippocampus and in in vitro part we cultured hippocampus neurons (in 7 groups: control, 3 dose of aqueous extracts of rosemary and 3 dose of aqueous-alcoholic extracts of rosemary) and then cell viability was assayed.
Results: We demonstrated that 200 mg/kg aqueous extracts of rosemary decrease the apoptosis gene expression and increase the anti-apoptosis gene expression in compare to ischemia (p<0.05) and decrease the neuron death in Hippocampal CA1 pyramidal neurons (p<0.05).
Conclusion: Present study demonstrated that cerebral ischemic tolerance induced by rosemary extracts pretreatment, the aqueous-alcoholic extracts of rosemary in 200 mg/kg dose was more effective to protect of hippocampus.