Assessment of different doses of nickel chloride on RBC, Hemoglobin and hematocrit in female Wistar rats
Assessment of different doses of nickel chloride on RBC, Hemoglobin and hematocrit in female Wistar rats
Zahra Azmoun,1Seyed Mohammad Hosseini,2,*Sohrab Kazemi,3
1. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran 2. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran 3. Cellular and Molecular biology research centre, health research institute, Babol University of medical sciences
Introduction: Nickel is one of the main heavy metals in the environment, which is present in the crust and core of the Earth. Although nickel is essential to many microorganisms and plants, if humans get exposed to a high amount of nickel, they might reveal different anomalies. As extensive quantities of studies have been implemented during recent years, numerous teratogenicity, Hemotoxic, and allergies that have been reported are attributed to nickel effects which people can be exposed through either gastrointestinal tract or inhalation. The current work was done to determine the impacts of sub-acute exposure with three doses of nickel chloride on some hematologic criteria in adult female Wistar rats.
Methods: 32 Wistar rats were used and distributed randomly into 4 groups, group one was considered as control which was administered 0.5 ccs of distilled water by intraparietal injection, and groups two three, and four obtained Nickel by doses of 10, 15, and 25 𝑚𝑔⁄𝑘𝑔 in order by intraperitoneal injection.
Results: Our findings exhibited that nickel chloride administration causes moderate alteration of complete blood count values in Wistar rats treated with 10 and 15 mg/kg dose while marked changes were seen in rats treated with 25mg/kg dose. Moreover, According to the levels of HCT and HGB of the 4th group, which are the lowest among all the groups, the dose 25 𝑚𝑔⁄𝑘𝑔 indicated as the most toxic dose in this investigation.
Conclusion: To sum up, we can conclude that these side effects are consequences of free radicals due to Nickel treatments and According to low levels of RBC, HCT, and HGB it ought to be reckoned that nickel has negative impacts on human and animal health and toxicity effect of nickel could be considered as one of them.