• Effect of Nickle chloride on element levels in the bone tissue of Wistar rats: calcium phosphor changes
  • Zahra Azmoun,1 Seyed Mohammad Hosseini,2,* Sohrab Kazemi,3
    1. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran
    2. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran
    3. Cellular and Molecular biology research centre, health research institute, Babol University of medical sciences


  • Introduction: Nickel (Ni) and its compounds are frequently used in industrial and commercial products such as stainless steel, alloys, and batteries. since human activities are the major cause of pollution, and metals can enter the food chain and bioaccumulate in hard and soft tissues/organs, which results in a long half-life of the metal in the body. The present review focuses on (Nickel II chloride ) and its effects on bone tissue. excess of this metal in the body can alter bone dynamics. Long-term exposure to high concentrations induces an imbalance in the bone mineral remodeling process. Different doses of Nickel are evaluated on element levels in the bone tissue in order to demonstrate these results.
  • Methods: 32 female Wistar rats were separated into 4 groups. The first group obtained water without treatment whilst three other groups received nickel by doses of 10, 15, and 25mg/kg, sequentially. All items were injected intraperitoneally and carried out thrice on days 8, 12, and 16. After 20 days, in order to evaluate biochemical values of calcium and phosphorus, blood samples were taken from every rat. Ultimately, rats were euthanized under general anesthesia by Ketamine and Xylazine.
  • Results: Although a dose-dependent increase in levels of calcium and phosphorus has been shown, the alterations between groups were not observable. Nevertheless, the calcium and phosphorus of the control group were at minimum levels. As nickel dose rises, calcium and phosphorus levels get higher. Thus, the highest levels belonged to nickel dose 25mg/kg treated rats.
  • Conclusion: Through this research, it can be manifested by elevated levels of calcium and phosphorus that nickel can cause destructive effects on rats, and kidney lesions should be considered as subsequences of nickel intakes.
  • Keywords: Nickel, Phosphor, calcium, bone tissue, Wistar Rat