The hsa-miR-192-5p miRNA of the ABCG2 genes can be involved in the development of HCC by acting on the signaling pathways of Bile secretion.
The hsa-miR-192-5p miRNA of the ABCG2 genes can be involved in the development of HCC by acting on the signaling pathways of Bile secretion.
Zahra Khital,1,*Maryam Khital,2Mohammad Rezaei,3Mansoureh Azadeh,4
1. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran 2. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran 3. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran 4. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
Introduction: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. HCC is the third leading cause of cancer-related death worldwide. Chronic hepatitis infection increases the risk of developing HCC. HBV increases HCC through direct and indirect mechanisms. The virus integrates its genome with the host and causes mutations in various cancer-related genes.
Over the years, new molecular methods have been developed to better understand and detect cancer early. These molecular methods, such as DNA microarray and PCR-based array, can show us molecular markers for early detection and prognosis of cancer. Large-scale gene expression profiles provide important insights into the biology of liver cancer. The results of these methods identified signaling pathways that could serve as therapeutic targets.
Methods: Data were extracted from GEO and then analyzed by R studio. The method of data analysis was differential expression. Finally, three genes were selected and their target miRNAs were found through miRwalk3.0. Then, by analyzing the interactions and pathways, appropriate miRNAs were selected.
Results: GSE121248 on Gene expression profiling of chronic hepatitis B induced HCC and adjacent-normal tissues was used. One of the genes found in this assay is ABCG2, whose miRNA is hsa-miR-192-5p. hsa-miR-192-5p is a miRNA related to the ABCG2 gene. This miRNA is abundant in the liver and is also involved in bile secretion. Exogenous levels can contribute to the prognosis of chronic hepatitis. induces hepatic trans-differentiation from human umbilical cord Wharton’s jelly derived mesenchymal stem cells.
Another selected gene is GTSE1 and one of its miRNAs is hsa-4524b-3p. This gene and its miRNA are effective in HCC with negatively regulated p53 expression. High expression is associated with tumor size and is associated with the development of HCC and increased proliferation of cancer cells.
The other selected gene is ITGA9 and its miRNA is hsa-miR-125b-3p. This gene is related to ECM-receptor interaction. Its expression is decreased in patients with HCC.
Conclusion: The ABCG2, GTSE1, and ITGA9 genes affect the progression of HCC disease by affecting Bile secretion, p53 signaling pathway, and ECM-receptor interaction in a ceRNA network.