Study of The effect of human Wharton's jelly mesenchymal stem cells extract on collagen situation in skin wound healing process in rats
Study of The effect of human Wharton's jelly mesenchymal stem cells extract on collagen situation in skin wound healing process in rats
Abdollah Abdollahpour,1,*Seyyed Esmael Safavi Khalkhali,2Bahram Amoghli Tabrizi,3
1. Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran 2. Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran 3. Department of Clinical Sciences , Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Introduction: A wound is a disorder in natural structure, purpose and its interior soft layer of skin. To maintain the integrity of skin is vital to prevent bleeding, inadequacy of water, and entrance of microorganisms. Skin lacerations are occurred in accordance of different cases such as physical, chemical and biological damages. The point of healing jagged part on our skin has been under the studying of scholars and researchers with minimum of side effect. Hence different ways and protocols have been proposed and tried with it. One of the most important factors in wound healing is the collagen situation. New fibroblasts begin to produce collagen very quickly, and this can be detected in wounds very early on the second day of injury. Collagen synthesis continues for some time after injury. Collagen production is an important part of wound healing process before and after the formation of new tissue. There has been new methods of treatment originates from stem cells which aimed to rebuild and heal some tissues which these cells shown their efficacy in healing animal disease too. Stem cells with their Prodigious capabilities of propagation and rehabilitation are unique in other kind of cells. Such an evident source of stem cells is the blood of Umbilical cord. The use of the cells in blood faced with some limits. Therefore the examined researches in this field are trying to use the product of the cells such as cells extraction. The current survey is aimed with the efficacy of Mysenchymal stem cells of Wharton jelly Umbilical cord to treat a mouse wound.
Methods: This experiment conducted in two levels of invitro and inviro. The first invitro step was to separate and seed stem cells of Wharton jelly Umbilical cord. After the last time of doing this and centrifuge we got solutions of Wharton jelly Umbilical cord. In invivo stage with 30 vistar mouse which were grouped in a pair of 5 groups with the first group (normal) second group (control) was jagged as 3 groups included extract of 5, 10, and 20% division used. This injection was performed interior tissue in 1 Cc around the wound with physiology serum group and with examination gp was cell extract. Jagged laceration was created by surgical punch after anaesthesia and epilation. After 21 days the death of mice some tissue models were gathered. The process of colouring by Van gison was conducted and histopolytical studies were taken into account. Also, some tissue models were gathered and proteins derived from the gene (Col-1α1) were examined by Western blotting.
Results: The results of this study showed In addition to faster healing of skin wounds in the treatment group, that the total intensity of collagen in all groups treated with cell extract was higher than the control group. The total intensity of collagen in the groups treated with cell extract was 10 and 20% significantly different from the control group. While the cell extract treatment group did not have a significant difference of 5% with the control group. It should be noted that the total intensity of collagen in the control group was significantly different from the healthy group (P <0.05). It should be noted that the highest total collagen intensity in the treatment groups is related to 20%.
Conclusion: More and more collagen synthesis promotes the healing of skin wounds. Therefore, increasing collagen in the cell extract treatment groups causes faster healing of skin wounds.