Houshang Nemati,1,*Mohammad hosein Ghahremani,2Sayed Hamid Madani,3Alireza firoz,4Ghobad Mohammadi,5Mehri Nazeri,6
1. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran 2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences 3. Department of pathology, Faculty of medicine, Kermanshah University of Medical Sciences 4. Faculty of Medicine, Tehran University of Medical Sciences 6. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Introduction: Psoriasis is a skin disease that causes red, itchy scaly patches, most commonly on the knees, elbows, trunk and scalp. Application and systemic delivery of small interfering RNAs offer many
effective therapeutic advantages for gene regulation in the skin.
Methods: In this study, the final concentration of 50 μM nano-curcumin at the time point of 48 h was chosen. Then we monitored suppression of EGF, EGFR, VEGF, IL8 and IL17 genes after treatment of A431 cells by SNA-NCs. The expression of genes was validated by qRT–PCR in A431 cells.
Results: Results showed that the curcumin-nanoparticles were stable and non-toxic. Experiments showed that curcumin conjugated with spherical nucleic acid gold nanoparticles can significantly reduce EGF, EGFR, and VEGF gene expression in cells.
Conclusion: Curcumin is a natural substance that is known to have anti-carcinogenic and anti-inflammatory effects against several types of cancers. A disadvantage of free curcumin is that it is highly hydrophobic and is poorly absorbed after oral administration.
It seems that, nano-curcumin has a direct anti-proliferative effect on A431 cell lines. We found that nano-curcumin significantly up-regulated the expression of the growth genes.