• Evaluation of SCAMP5 gene expression in the tissues of Iranian patients with glioblastoma multiforme
  • nasrin Karimi,1 Hamidreza Kheiri Manjilli,2,* Vajiheh Zarrinpour,3 Mohammadmehdi Forghanifard,4
    1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
    3. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
    4. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran


  • Introduction: Glioblastoma multiforme (also called glioblastoma, GBM) is uncommon cancer that accounts for more than half of the primary malignant brain tumors diagnosed. The study of the expression of genes involved in glioblastoma can be effective in early detection, so we discussed the clinical significance and biological role of the SCAMP5 gene transcript in glioblastoma cancer, which is unknown.
  • Methods: In the present study, 25 samples of healthy tissue margin of glioblastoma multiform were used as a control group and 25 samples of glioblastoma multiforme tissue as a patient group were obtained before treatment and RNA extraction and cDNA synthesis were performed. SCAMP5 expression levels were assessed by Real time-PCR. ACTB was used as the internal control. Statistical analysis was performed by GraphPad Prism v.8.0.1.
  • Results: Down-expression of SCAMP5(P<0.05) in glioblastoma multiforme tissue samples was significant compared to healthy tumor margin tissue. Expression of SCAMP5 gene with age with P-value = 0.0024 is statistically significant. But the expression of this gene with gender with P-value = 0.4611 is not statistically significant. Decreased expression of the SCAMP5 gene can play an important role in glioblastoma cancer.
  • Conclusion: SCAMP5 was expressed in tumor tissue of patients with GBM lower than healthy tissue around the tumor. According to this study, it may be possible to use the expression of this gene as a marker as an early diagnostic method, but needs further study.
  • Keywords: Glioblastoma multiforme, SCAMP5, Real time-PCR, expression