• The role of mir-320 in the Bladder cancer cell line
  • Mahshid Damandan,1,*
    1. Center for Cell Pathology Research, Department of Life Sciences, Khazar University, Baku, Azerbaijan.


  • Introduction: Bladder cancer (BC) is one of the most common cancer in both women and men in all over the world. MicroRNAs are involved in multiple processes such as cell differentiation, transcription, inflammation, proliferation, cell signaling, and apoptosis. In molecular biology mir-320 microRNA is a short RNA molecule. Therefore, this study was performed to investigate the biological functions and molecular mechanisms of miR-320 in human bladder cancer cell line.
  • Methods: The bladder cancer cell line was retained in the RPMI 1640 medium, supplemented with 10% FBS and cultured under standard conditions of 95% moisture and 5% CO2 at 37°C. Transfection of miR-320 mimic and negative control (NC) was established using Electroporation technique. The tetrazolium-based MTT assay has long been regarded as the gold standard of cytotoxicity assays as it is highly sensitive and has been miniaturized for use as a high-throughput screening assay. MTT assay was performed to investigate the cytotoxic effect of miR-320 mimic EJ138 cell line. Cytotoxic and cell viability performed using MTT method, the optimum of which was determined to be 40.
  • Results: These findings indicate that transfected miR-320 mimic could suppress EJ138 cell line proliferation and Infected EJ138 cell line containing miR-320 mimic administration with MTT assay showed that the proliferation rates of cell line decreased.
  • Conclusion: These results support the tumor-suppressive effect of miR-320 in the EJ138 Bladder cancer cell line by reducing proliferation, Therefore, miR-320 may be a novel molecular therapeutic target for the treatment of BC.
  • Keywords: microRNA-320, Bladder cancer, cytotoxicity, electroporation and MTT assay.