• Determining the relationship between nsSNPs genome sequencing in human SLC2A2 gene and phylogenetic function of nucleotides
  • Alam Ara Gholami,1 Ali Ahmadi,2,*
    1. Assistant Professor, Department of Biological Sciences and Technologies, Islamic Azad University, Sari Branch, Sari, Iran
    2. BSc. Student, Department of Biological Sciences and Technologies, Islamic Azad University Sari Branch, Sari, Iran


  • Introduction: DNA sequencing (DNA) is a process in which the sequence of nucleotides in a DNA molecule is determined. The DNA of living organisms consists of a special sequence of nucleotides. DNA sequencing allows scientists to examine the relationships between organs and their phylogenetic relationships by comparing DNA between different organs. Glucose transfer through the eukaryotic cell membrane is carried out by members of the glucose transporter family (GLUT), which are mainly divided into three classes (I, II and III) based on phylogenetic relationship. In humans, a class I member called GLUT2 is encoded by the carrier family of salt 2, the facilitated glucose transporter 2 gene (SLC2A2) on the third chromosome. The requirement for DNA sequencing was first proposed by Francis Crick's theory. Protein-mediated movement of glucose in cell membranes is made possible by GLUT2, a transmembrane transporter protein. Regulates glucose uptake and insulin secretion into pancreatic cells. Modern DNA sequencing uses powerful methods that allow DNA sequences to be identified in a very short time. This technology has made it possible for many companies to offer their customers methods for home DNA testing. An example of DNA sequencing DNA sequencing In the past and in the early years of the invention of this technology, it took a long time and sometimes several years to achieve the result, but now with the development of technology, DNA sequencing can be done in a few In addition to DNA sequencing, services such as DNA sequencing also offer services such as single-nucleotide polymorphism tests to identify and study genetic changes and mutations in the human genome. Sequencing Target Areas although whole genome sequencing using NGS genome techniques is an optimal and community-based method, it is not available in many research and clinical laboratories. The aim of this study was to determine the relationship between genome sequencing and phylogenetic function of nucleotides
  • Methods: This study was a secondary study with a narrative approach approach that in 2022 by searching for keywords such as sequencing, NGS, genome, phylogenetics, nucleotides and mutations in valid databases such as Scopus, Sciences Direct, Web of Sciences and PubMed. All input and output criteria of the study were examined. In this study, 15 articles were selected, of which 10 articles were included in the study
  • Results: There are 13 extracellular domains, 12 interstitial domains and 5 cytoplasmic domains in human GLUT2. The risk of Fanconi-Bickel syndrome (FBS), diabetes, breast cancer (BC) and Alzheimer's disease (AD) is associated with poor GLUT2 function. The most common form of genetic mutation is single nucleotide polymorphism (SNPs). Non-synonymous SNPs (nsSNPs) can lead to amino acid changes and subsequent changes in phenotype. In this study, intra-silicon analysis was performed to find the phylogenetic relationship of human GLUT2 protein and the potential adverse effects of nsSNPs in its coding region. Identification of different variants using sequencing of the whole genome is of particular importance to the identification of genetic variants of a particular trait. Variants that make some people more susceptible to the disease A study of the entire genome through sequencing was initially performed on variants that are common in the community, but with NGS methods and sequencing of the entire genome, it is possible to study variants with abundance. Provided less.
  • Conclusion: Using this method, the binding site of some transcription factors and genes under its control can be identified. The Methyl-Seq technique is also used to map the genome methylation pattern and to identify regions that are differentially methylated.
  • Keywords: sequencing, NGS, genome, phylogenetics, nucleotides and mutations