Introduction: The human body is made up of different tissues and these tissues are vulnerable. Today, we use tissue transplantation to treat some of these injuries. For tissue transplantation, we need cells that are strong on the scaffolds. We have used PCL scaffolding in this project. PCL scaffold is a type of polymer that consists of repeating units of hexanovate. PCL scaffold is inherently biodegradable and is widely used in drug delivery networks today. Graphene oxide is a carbon allotrope that is hydrophilic and has high solubility and is used in drug delivery and anticancer drugs. Also, silver nanoparticles have antibacterial properties and are used in wound dressings.
Methods: To carry out this research, first, graphene oxide was synthesized and silver nanoparticles were prepared and electrospun in PCL scaffold. Structure of nanofibers in The electron microscope was examined and then fibroblast cell culture was performed on the nanofiber scaffold. At the end of the life test Compatibility of MTT and DAPI staining was performed and examined with a fluorescent microscope.
Results: The results showed that the supramolecular PCL nanofibers coated with a mixture of graphene oxide and silver with a higher density were more similar to the natural ECM of the body and has shown a better ability to support cell growth and proliferation
Conclusion: The results showed that the supramolecular PCL nanofibers coated with a mixture of graphene oxide and silver with a higher density were more similar to the natural ECM of the body and has shown a better ability to support cell growth and proliferation