Introduction: Fungi, as one of the most important plant pathogenic agents, are the main source of bioactive secondary metabolites, and they are of significant importance in environmental interactions, among which metabolites can be antibiotics, toxins, and enzymes.
Methods: In this research, the antimicrobial properties of the secondary metabolite isolated from Fusarium were investigated against the Gram-negative Escherichia coli bacteria.
Results: The results of the microbial tests showed that the metabolites extracted from the Fusarium fungus had an antibacterial effect on Escherichia coli bacteria with a concentration equivalent to 31.25 microliters. per milliliter. Regarding the minimum lethal concentration of bacteria by fungal metabolites, it was found that the metabolites extracted from Fusarium mushroom had a concentration equivalent to 62.5 microliters/ml. In the test to determine the antibacterial property using the disk method, it was found that the concentration of 15.6, 31.25 and 62.5 microliters/ml of the Fusarium mushroom metabolite could create a non-growth halo with a diameter of 6, 13.91 and 17.13 microliters/ml.
Conclusion: According to the obtained results, it can be claimed that the use of fungal metabolites to control the growth or even the death of bacteria can be useful as an alternative to antimicrobial chemical compounds.