• Graphene-based biosensor for specific detection of ovarian cancer cells
  • Narjes Hafezi,1 Zeinab sadat jalali,2,* Samin Azarnezhad Gargari,3 Aylin movahedyfar,4 Sahar Barjasi,5
    1. Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
    2. Jahrom university of Medical Sciences, Jahrom,Iran
    3. Tabriz university of Medical sciences, Tabriz, Iran
    4. Tabriz university of Medical sciences, Tabriz, Iran
    5. Rafsanjan university of Medical science, Rafsanjan, Iran


  • Introduction: Ovarian cancer (OC) is nominated as one of the most aggressive gynecologic malignancies and is the main cause of gynecological malignancy-related mortality worldwide. So, developing an efficient early detection method for OC via a facile and low-cost approach is vital. Considering that graphene oxide has high biocompatibility, it is used as a promising new material at the forefront of material design for biomedical applications. Our goal in this study was to peruse the function of Graphene-based biosensors for specific detection of ovarian cancer cells.
  • Methods: For this research, existing articles in PubMed, Web of Science, Magiran, Sid, and Google Scholar databases that have been published till 2022 are systematically selected, and 16 articles are included in this study. This research is done in English considering the following keywords: Graphene-based biosensor, ovarian cancer, Cancer cells, and Graphene oxide.
  • Results: Due to the high water affinity and single-stranded DNA (ssDNA) sorption characteristics of GO, a GO-based qRT-PCR assay for the detection of miRNAs dependent on OC was designed and developed. In the GO-based qRT-PCR system, GO could significantly improve the sensitivity and specificity of the qRT-PCR assay by noncovalently interacting with primers and ssDNA and reducing the occurrence of non-specific amplification. Moreover, the detection of miRNAs associated with OC confirmed that GO-based qRT-PCR assay could differentiate benign ovarian tumors from OC.
  • Conclusion: Generally, findings provide robust evidence that surprisingly graphene-based flexible biosensors demonstrate highly sensitive and can be effectively used as a promising method for screening and improving the overall survival of patients with OC, but more studies are needed.
  • Keywords: biosensor, Ovarian Neoplasms, Neoplastic Stem Cells, Graphene oxide