Introduction: Bone tissue engineering requires a well-designed scaffold that can be biodegradable, biocompatible, and support the stem cells to osteogenic differentiation.Biodegradable polymers, such as polycaprolactone (PCL) and Poly(L-lactide)(PLLA) , are promising materials in the field of tissue engineering and regenerative medicine, which aims at creating viable options to replace permanent orthopedic implants. In this study we evaluate the osteogenic differentiation and gene expression of human bone marrow mesenchymal stem cells (hBMSCs) into PCL and PLLA scaffolds
Methods: MSCs were cultured on PCL and PLLA scaffoled for 21 days. Expression of osteoblast-related genes, including ,Runt-related transcription factor 2 (Runx2), osteonectin and collagen type1 , was assessed by real time-PCR.
Results: The real time PCR showed that the osteogenic gene expression including osteonectin , collagen type 1 and runx2 seeded in the nanfiber culture has higher expression than cells differentiated in control culture.
Conclusion: It can be concluded that PLLA and PCL are suitable substrates to support the proliferation and osteogenic differentiation of MSCs and holds promising potential for bone tissue engineering and regenerative medicine applications
Keywords: PLLA, PCL, Bone tissue engineering, gene expression. scaffold