• Bioinformatics analysis of hsa-miR-3615 and hsa-miR-302c-3p as novel candidates for the regulation of SSBP1 overexpression in Glioblastoma
  • Pegah Kamalinia,1 Mohammad Rezaei,2 Mansoureh Azadeh,3,*
    1. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran.
    2. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    3. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran


  • Introduction: Brain cancer is almost a rare disease, but Glioblastoma(GBM) is the most common type of brain cancer, accounting for about 15% of primary brain tumors, which is considered one of the most hazardous cancers in the world. [1]in this analysis, we examined disease-related genes with over-expressed in GBM cancer to discover miRNAs that provide possible biomarkers and methods to improve the treatment and control of this disease. Therefore, we have targeted the bioinformatics analysis of the pathways of these genes and the influencing factors on their expression control.
  • Methods: we acquired the gene expression data with the comparison of 34 GBM tumors and 13 control samples (GSE50161) from the NCBI Gene Expression Omnibus(GEO) (http://www.ncbi.nlm.nih.gov/geo/). In this analysis, all genes that were over-expressed in GBM have been checked with the criteria of a threshold value of |logFC| >1 and an adj.P-value< 0.05. The pathways of genes were checked by the Kegg section in the enrichr database. [2][3] In addition, by using the miRWalk database, we noticed the interactions of our target genes with the related microRNAs, which determined the position and role of our found data (Score: 1.00)[4].
  • Results: Single Stranded DNA Binding Protein 1 gene selected in GEO2R analysis with adj.P.Val= 6.05e-11 and a threshold value of |logFC|=1.57. In continuance of the process, using UniProt[5], geneCard[6], and EnrichR[7], we understood that SSBP1 plays a very important role in the positive regulation of DNA-dependent DNA replication (GO:2000105) and DNA unwinding involved in DNA replication (GO:0006268). By analysis of possible miRNA-mRNA interactions discovered hsa-miR-3615, hsa-miR-302c-3p as significant targets for SSBP1 with negative free energy and worthy align score were found. [8] As well as our further analyzes by LncBase v.3[9] in relative to the role of lncRNAs with our target microRNAs, we discovered the role of SNHG1, HOTAIR, H19 to express several lncRNAs to interact with our target miRNAs.[10]
  • Conclusion: Brain cells become carcinomatous and escape from apoptosis by overexpressing SSBP1. Our predicted ceRNA network including the mentioned LncRNA and miRNA can regulate the development and metastasis of GBM by affecting the expression level of SSBP1 by regulating the cell cycle.
  • Keywords: Glioblastoma cancer, miRNA, LncRNA, ceRNA