• MicroRNAs-199a-5p, 3120d, 215,194-1,664a prevent development of cancer cell and tumor spread by directly targeting oncogenic genes in Esophageal squamous cell carcinoma
  • Setare Samizade,1,* Alireza Nasr Esfahani,2
    1. Islamic Azad University, Department of Biology, Falavarjan Branch, Isfahan, Iran
    2. Islamic Azad University, Department of Biology, Falavarjan Branch, Isfahan, Iran


  • Introduction: Esophageal squamous cell carcinoma (ESCC), is the most common type of esophageal cancer worldwide, mainly occurring in the Asian esophageal cancer. Phosphatidlinositol 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway is one of the most important cellular signaling pathways, which plays a crucial role in the regulation of cell growth, differentiation, migration, metabolism and proliferation. MicroRNAs, are small noncoding RNAs which regulate gene expression primarily at the posttranscriptional level and their expression profile improve classification diagnosis and prognostic information of malignancies including ESCC cancer as oncogene or tumor suppressor. Here in, this study investigates the communication between mir-199a-5p, 3120d, 215, 194, 664a and ESCC cancer in cellular pathways.
  • Methods: By using mirbase, miRNA properties were obtained. The mirtarbase and MIRWALK2.0 target genes were identified. Through NCBI, the expression of target genes in the normal and ESCC tissue was obtained. Using DAVID, signal paths were obtained and the pathways associated with ESCC were interpreted. The gene network was obtained through GENE MANIA.
  • Results: The result demonstrated that mir199a-5p, 3120d, 215, 194 and 664a inhibit ERK by blocking Ras, SOS which active Raf, MEK through phosphorylation. So ERK by blocking ELK, MYC effect on adhesion increasing through preventing cell migration and suppress cancer. Mentioned microRNA by inhibiting EGFR, Ras, SOS, ERK inhibit proliferation of cancer cell and tumor spread. MicroRNAs by inhibiting Ras, PI3K take their stimulation impact of PIP3 and block Act, IKK, NFkB and prevent cell migration and metastasis.
  • Conclusion: Mir199a-5p, 3120d, 215, 194 and 664a effect on metastasis, proliferation and adhesion of cancer cell. Mentioned microRNAs by effecting on Act, Ras, SOS, EGFR, MAPK1, PI3K in ERB, Non-small cell ESCC cancer, RAS, PI3-ACT pathways, inhibit proliferation and metastasis by effecting on adhesion increasing and prevent cell migration. Therefore MicroRNAs act as tumor suppressor and destroy cancer by preventing the development of cancer cell and spread of tumor.
  • Keywords: Esophageal cancer, Signaling pathways, MicroRNA, Oncogenic genes