• Detection of tetracycline using aptasensors
  • Meysam Hesari,1 Mahdiyeh Nasiri,2 Erfan Ghalenoei,3 Mehrana radaei,4 Seyed Sajad Oliaee,5 Abed Ebrahimi,6,*
    1. Hamedan university of medical sciences
    2. Shahid sadoughi university of medical sciences
    3. Lorestan university of medical sciences
    4. Tehran university of medical sciences
    5. Shahid Sadoughi university of medical sciences , Yazd , Iran
    6. Department of Operating Room , School of Allied Medical Sciences


  • Introduction: Tetracycline is a broad-spectrum antibiotic used in the therapy of human and animal. Residual tetracycline in food are often used as model compounds to develop aptasensors. Until now, more than 100 advanced aptasensors toward tetracycline have been developed. The purpose of this research is to investigate recent advances in aptasensors for detection of tetracycline
  • Methods: In the forthcoming systematic review, the required data were collected using keywords and citing valid databases such as Scopus, PubMed, Google Scholar and ProQuest. The statistical population includes all studies conducted until 2022 in the field of Detection of tetracycline using aptasensors. After reviewing the relevant findings and evaluating the quality of the data, 18 articles were analyzed.
  • Results: After the TET aptamer modified onto multi-walled carbon nanotubes (MWCNTs-CS)/ interdigital array microelectrode (IDAM), the EIS presents an apparent increase, which was due to the inhibition effect of the macromolecules for electron transfer. After incubated with TET, the aptasensor has a significant change in impedance, but the non-specific oligonucleotide modified sensor almost stayed unchanged. The main reason lay in that the oligonucleotide cannot be combined with TET and TET cannot be bound to the electrode to prevent electron transfer, leading to the invariability in the impedance value.
  • Conclusion: Antibiotics are widely used and cause the development of antibiotic-resistant bacteria, allergic reactions and various side effects on human health. Therefore, ultrasensitive assays for the detection of tetracycline are of great interest. Such advanced aptasensors promise to overcome many challenges as biosensors are highly sensitive, easy to operate, and portable.
  • Keywords: Tetracycline, Aptasensor, Biosensor