• Evaluate the effect of co-treatment of Alpha-Lipoic Acid and Injectable platelet rich fibrin (i-PRF) bioscaffold on inflammation in mouse autotransplanted ovarian tissue
  • sahar hatami,1,* Seyed Mohammadali Shariatzadeh,2 Malek Soleimani Mehranjani,3
    1. Phd student.Department of Biology, Faculty of Science, Arak University, Arak, Iran.
    2. Department of Biology, Faculty of Science, Arak University, Arak, Iran.
    3. Department of Biology, Faculty of Science, Arak University, Arak, Iran.


  • Introduction: Ovariaan tissue transplantation preserve fertility in patiant young woman and girls after chemotherapy and radiotherapy. nevertheless, ischemia-reperfusion (I/R) injury occurs during the early post-transplantation days leads to increased inflammation and eventually damage to autografted tissue. Injectable platelet rich fibrin (i-PRF) is a liquid formulation of platelet rich fibrin (PRF) without the use of anti-coagulants. i-PRF is bioscaffold and contains, leukocytes, circulating stem cells, platelets and growth factors. Alpha-lipoic acid (ALA) is also a strong free radical scavenger with anti-inflammatory properties. we aimed to research the effect of co-treatment i-PRF bioscaffold and ALA on the serum level of inflammatory factors such as interlukin 6, 10 (IL6,10) and Tumor necrosis factor-α (TNF-α) in the mouse ovarian tissue following transplantation.
  • Methods: Mice were divided into three groups: control, autograft + saline (whole ovarian tissue transplanted in the gluteus superficialis muscle, saline directly injected into it), autograft + i-PRF+ ALA (first, the mice received 100 mg/kg" intraperitoneal injections of ALA, 30 minutes before transplantation than whole ovarian tissue transplanted in the gluteus superficialis muscle, i-PRF was directly injected into it ). Serum concentrations of IL-6, IL-10 and TNF-α were measured 7 days after ovary transplantation. Then the obtained data analyzed using one-way ANOVA and Tuckey’s test and the means were considered significantly different at p-value < 0.05.
  • Results: The serum level of TNF-α and IL-6 in the autograft group increased significantly compared to the control group , while it showed a significant reduction in the autograft + i-PRF+ ALA group compared to the autograft group (p < 0.05). Moreover, Serum concentrations of IL-10 was significantly lower in the autograft group when compared to the control group, Whereas it showed a significant increase in the autograft + i-PRF+ ALA group compared to the autograft group (p < 0.05).
  • Conclusion: we results revealed that co-treatmentr i-PRF bioscaffold and ALA can Improve ischemic injuries in transplanted ovaries through decrease inflammatory factors.
  • Keywords: Ovarian tissue transplantation, α-lipoic acid (ALA). Injectable platelet rich fibrin (i-PRF). Ischem