• Investigation Of The Histological Effects Of Phytoestrogen in Cuminum cyminum L . On Ovarian Tissue of Three-Spot Gourami (Trichogaster trichopterus)
  • Tahereh Naji,1,* Homayoon Hosseinzadeh Sahafi,2 Anahita Attarhamidi,3 Ziba Kolouei,4
    1. Department of Basic sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
    2. Iranian Fisheries Science Research Institute, Agricultural Research and Education Organization, Tehran, Iran.
    3. Department of Basic sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
    4. Department of Basic sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.


  • Introduction: Different types of plants produce compounds that can mimic or interfere with the effects of estrogen hormones in animals. These compounds, called phytoestrogens, are weaker than normal estrogens and are found in medicinal plants. Phytoestrogens are non-steroidal compounds derived from plants that have biological activity similar to estrogen. Studies indicated that phytoestrogens in Cuminum cyminum L could have affected on ovarian tissue. The aim of this study was to investigate the effects of Cuminum cyminum L. of methanolic extract on ovarian tissue of Trichogaster trichopterus.
  • Methods: In this study, sixty pieces of Trichogaster trichopterus were randomly divided into 6 groups, including two control groups and treatment group were divided into 4 groups. All fish injected with 10, 20.30 and 50 mg/kg doses of the extract of Cuminum cyminum L to IM under every other day for 20 days. After the exposure period, the fish were anesthetized with extract of clove flower . The length and weight of fish were measured and the ovarian tissues of fish have been histology studied. Then, ovarian tissue compared with control and treatments groups.
  • Results: The administered of Cuminum cyminum L plant extract with different doses have not increasing effect on body weight and ovarian structure. Administration of the extract was not significant change in volume and size of ovarian and follicles in the treated group compared to the control group. Ovarian weight of treated group was indicated a slightly decrease. Histologic studies showed morphological changes in the ovarian tissue. The results showed that using of Cuminum cyminum L plant extracts with conventional doses had significant adverse effect on the ovarian tissue of Trichogaster trichopterus.
  • Conclusion: The analysis of the results obtained from the comparison of the mean percentage of gonadosomatic index between different treatments and also the histological changes from different groups in terms of the dominant developmental phase of oocytes in each group showed that the use of Cuminum cyminum L plant extract can cause maturation oocytes in three spot gourami fish.
  • Keywords: Cuminum cyminum L., Trichogaster trichopterus, Ovarian tissue