• 58S scaffolds coated with Zein for bone regeneration
  • faezeh esmaeili ranjbar,1,* Afsaneh Esmaeili Ranjbar,2 Vajihe Taghdiri Nooshabadi,3 Elham Hasanzadeh,4
    1. Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
    2. Emergency Medicine Department, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
    3. Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran
    4. Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran


  • Introduction: Bone defects are generated by fractures, trauma, congenital bone malformations, skeletal disorders, and tumor removal. Tissue engineering is an innovative method of producing tissue grafts. 58S is a bioactive glass made up of three components: 58% SiO2, 38% CaO, and 4% P2O5. Zein is FDA- approved polymer, it is natural, biodegradable, and biocompatible, and it has the ability to assist bone regeneration. In this study, we prepared a porous scaffold of 58S bioactive glass and coated with Zein and evaluated bioactivity, porosity, and cell adhesion.
  • Methods: Bioactive glass scaffolds were created using the replica plating method, subsequently, the manufactured scaffolds were coated with Zein (7%w/v) via immersion. Coated scaffolds were characterized with the scanning electron microscope, and morphology, cell adhesion, and bioactivity of them were evaluated. The porosity of coated scaffolds was estimated via the Archimedes procedure.
  • Results: The SEM results showed that cells adhered and proliferated on coated scaffolds. In addition, SEM images of bioactivity assessment revealed that the Hydroxycarbonate apatite crystals were deposited on prepared scaffolds. Moreover, the Porosimetry experiment presented that coated scaffold's Porosity was around 60 %.
  • Conclusion: the acquired results demonstrated that the manufactured scaffold is biocompatible, bioactive, and porous and could be a suitable candidate for bone regeneration
  • Keywords: bone tissue engineering, bioactive glass, Zein, scaffold