مقالات پذیرفته شده در ششمین کنگره بین المللی زیست پزشکی
CCL21 and HSPA1A differentially expressed in ischemic heart disease
CCL21 and HSPA1A differentially expressed in ischemic heart disease
Mahshid Malakootian,1,*Akram Gholipour,2
1. Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences 2. Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences
Introduction: Myocardial ischemia occurs when blood flow to the myocardium obstructed by a partial or complete blockage of a coronary artery by atherosclerosis. Rupture of plaques lead to myocardial infarction. The aim of this study is to identify important differentially expressed genes and their regulatory miRNAs in ischemic heart disease.
Methods: The expression profiling array of adipose tissue of 5 patients and 5 Control samples were obtained from the GEO database (GEO accession: GSE 152326), and the samples were analyzed. Genes with differential expression patterns were isolated with the GEO2R by P.value < 0.05 and logfoldchange (LogFC) # 1 investigation. In addition, miRNAs targeted 3´-UTR of selected genes were examined through miRcode database.
Results: The results showed that 3744 genes had differential expression among the samples in which C-C motif chemokine ligand 21 (CCL21) gene was the highest downregulated gene with logFC = -4.607 and p.value= 0.00001978. Heat shock protein family A (Hsp70) member 1A (HSPA1A) gene was the highest upregulated gene with logFC = 2.369 and p.value= 0.00078007. Further, miR-138 with two and miR-146 with one target sites might have regulate the expression of CCL21 and HSPA1A, respectively.
Conclusion: Our analysis showed that analysis of differentially expressed genes and 3´-UTR target sites related microRNAs in adipose tissue of ischemia could help to find accurate biomarker.