Comparison of the effect of liposomes of Peppermint extract and Cinnamon essential oil against Staphylococcus aureus with beta-lactamase gene isolated from packaged minced meat in Yazd
Comparison of the effect of liposomes of Peppermint extract and Cinnamon essential oil against Staphylococcus aureus with beta-lactamase gene isolated from packaged minced meat in Yazd
Ruholla rahimi nasab,1mostafa rahimi nasab,2Mohammad Javad Forouzani-Moghaddam,3,*
1. Bachelor of Biotechnology, Yazd University, Yazd, Iran 2. Bachelor of Biotechnology, Yazd University, Yazd, Iran 3. Msc of Food Microbiology, School of Paramedical, Iran University of Medical Sciences, Tehran, Iran
Introduction: Staphylococcus aureus is one of the most important pathogenic microorganisms in meat products, especially those that come into contact with the hand during repeated production. Beta-lactamase drugs, especially the new generation of Cephalosporins, is used to treat most infections caused by Staphylococcus aureus, but the production of beta-lactamase by some strains has failed to treat infections associated with the organism.
The aim of this study was to evaluate the degree of contamination and compare the antimicrobial effect of liposomes Peppermint extract and Cinnamon essential oil on Staphylococcus aureus with beta-lactamase gene isolated from minced meat in Yazd.
Methods: For this purpose, sampling of 45 packaged meat distribution and supply centers in Yazd city was performed under hygienic conditions and all samples were tested for the presence of Staphylococcus aureus with beta-lactamase gene tested by biochemical methods and molecular confirmation with PCR test. Were placed. The antibacterial effect of the liposomes of red pepper extracts and red onion on the mentioned isolates was evaluated with the tests of minimum inhibitory effect (MIC), minimum lethal effect (MBC), well release and bacterial growth curve.
Results: The results showed that 17% of the samples were infected with Staphylococcus aureus bacteria with the beta-lactamase gene. The liposomes of Peppermint extract and Cinnamon essential oil showed a good antibacterial effect against these isolates, and in all tests, the liposomes of Cinnamon essential oil was more effective than Peppermint extract
Conclusion: By proving the stronger antimicrobial effect of Cinnamon essential oil liposomes, it is recommended that in dishes such as grilled types of meat prepared from minced meat, be sure to use the liposomes Cinnamon essential oil.