Evaluation of the frequency of oxa-23, oxa-58 and ndm genes in clinical samples of Acinetobacter baumannii by Multiplex-PCR method
Evaluation of the frequency of oxa-23, oxa-58 and ndm genes in clinical samples of Acinetobacter baumannii by Multiplex-PCR method
Atena Salman Hormati,1Mohsen Mirzaee,2Reza Yari,3,*
1. MSc of Cell and Molecular Biology, Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran. 2. Assistant Professor, Department of Laboratory Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran. 3. Assistant Professor, Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.
Introduction: Acinetobacter baumannii is the cause of a wide range of nosocomial infections. Antibiotic resistance of this organism is a major challenge worldwide. The aim of this study was to identify oxa and ndm genes by multiplex-PCR and to determine the pattern of antibiotic resistance.
Methods: This study was performed in a period of 8 months by collecting 25 bacterial plate isolates. Antibiotic susceptibility testing was performed on Müller Hinton agar medium by disk diffusion method. MPprimer software was used to design the primers. After data collection, the level of significance was assessed at P˂0.05 using SPSS 22.
Results: Except for gentamicin, cefpime, ciprofloxacin and tobramycin, there is a significant relationship between resistance pattern and sensitivity. All Acinetobacter baumannii isolates were sensitive to ceftazidime. Also, 20% of the isolates were resistant to imipenem, which are considered carbapenem-resistant isolates of Acinetobacter baumannii. Of the 25 isolates studied, 5 isolates (20%) had oxa58 gene, 5 isolates (20%) had oxa23 gene and three isolates (12%) had ndm gene.
Conclusion: The results showed a high widespread of antibiotic resistance among Acinetobacter baumannii isolates, which emphasizes the need to develop programs in the control and treatment of this powerful pathogen. Also, the frequency of beta-lactamase-producing isolates in hospital isolates has been growing, which indicates the need for more attention of health centers in prescribing drugs.