Investigation of the effect of antibacterial nano-lipid system containing Rosemary essential oil
Investigation of the effect of antibacterial nano-lipid system containing Rosemary essential oil
Faezeh Askariyan,1,*
1. Department of Biology, Faculty of Science and Engineering, Science and Arts University, Yazd, Iran
Introduction: The aim of this study was to investigate the antibacterial effect of nano-lipid system containing Rosemary essential oil. For this purpose, the lipid system containing Rosemary essential oil has been synthesized for gram-positive (Staphylococcus aureus) and gram-negative (E.coli) antibacterial methods.
Methods: The type of study is laboratory research. The nanoparticle synthesis method is Mozaffari method. Particle characterization has been performed in terms of size and charge with DLS and morphology with the Atomic Force Microscope (AFM) and the amount of loading and release with the spectrophotometer. MIC tests were then performed to evaluate the performance of nanoparticles containing Rosemary essential oil on Staphylococcus aureus and Escherichia coli.
Results: The average particle diameter was 63 nm and its zeta potential was -16.7 mV. The loading rate in nanoparticles was 63%, which was calculated by reading the absorption of light from the standard Rosemary curve. The minimum inhibitory concentration (MIC) of Staphylococcus aureus and E.coli for nanoparticles was 15.625 and 31.25 mg / ml.
Conclusion: Nanoparticles containing Rosemary essential oil kill gram-positive (Staphylococcus aureus) and gram-negative bacteria (E.coli) and can be used as antibacterial nano-systems.