Analysis of the expression of selected miRNAs (miR-17-5p and miR-20-5p) and LncRNAs (LINC01605 and FAS-AS1) involved in the apoptosis signaling and their target gene, Caspase 3 in glioblastoma multiform
Analysis of the expression of selected miRNAs (miR-17-5p and miR-20-5p) and LncRNAs (LINC01605 and FAS-AS1) involved in the apoptosis signaling and their target gene, Caspase 3 in glioblastoma multiform
Shirin Setoodeh Haghighi,1Nasrin Motamed,2,*Sayyed Mohammad Hossein Ghaderian,3
1. 1Department of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran 2. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran 3. Skull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Introduction: Glioblastoma multiform (GBM) is an invasive cancer, that causes high mortality in patients. Disruption of the apoptosis process is one of the main pathogenesis of the disease. Recently, LncRNAs and miRNAs have been shown to play an important role in the process of apoptosis. In this study, we examined the expression changes of miRNAs (miR-17-5p and miR-20-5p) and LncRNAs (LINC01605 and FAS-AS1), as well as Caspase 3 in GBM patients.
Methods: In this study, 100 patients participated in two groups of 50 individuals, including 50 GBM patients as the case group, and 50 healthy individuals as the control group. Using RT-PCR, the expression changes of miRNAs (miR-17-5p and miR-20-5p), LncRNAs (LINC01605 and FAS-AS1) and Caspase 3 were examined in both groups.
Results: Expression of LINC01605, miR-20-5p and miR-17-5p increased in patients, while expression of Caspase 3 and FAS-AS1 decreased; the difference was statistically significant between the two groups. In addition, it was found that these factors have the appropriate sensitivity and specificity as diagnostic markers. In order to evaluate the diagnostic value of CASP3, LncRNAs (LINC01605 and FAS-AS1) and miRNAs (miR-17-5p and miR-20-5p), the receiver operating characteristic (ROC) curves were performed. Analysis showed, that there was an indirect relationship between the expression of miR-20-5p and miR-17-5p with patients’ sex, however, no significant relationship was observed between them. An indirect relationship was also found between patients’ gender. However, there was a direct relationship between FAS-AS1 and patients gender, but no significant relationship was observed between them (P > 0.05). Then the Area Under the Curve (AUC) values were analyzed. ROC curve analysis of genes showed the following information (Sensitivity: 92% and specificity: 64% for Caspase 3, Sensitivity: 40% and specificity: 84% for LINC01605, Sensitivity: 60% and specificity: 88% for FAS-AS1, Sensitivity: 94% and specificity: 60% for miR-17-5p, and Sensitivity: 88% and specificity: 74% for miR-20-5p). These genes can be used as diagnostic biomarkers to discriminate GBM patients from control subjects.
Conclusion: Finally, LINC01605, FAS-AS1, miR-20-5p, miR-17-5p and Caspase 3 can be used as apoptosis predictors in the GM patients.