• Isolation and Identification of Halophilic Bacteria Producing Asparaginase Enzyme from Howz-Sultan Salt Lake
  • Mohsen Sheykhhasan,1 Azar Sheikholeslami,2 Hoda Fazaeli,3 Faezeh Davoodi,4 Naser Kalhor,5,*
    1. Department of Mesenchymal Stem Cells, the Academic Center for Education, Culture and Research, Qom Branch, Qom-Iran.
    2. Department of Mesenchymal Stem Cells, the Academic Center for Education, Culture and Research, Qom Branch, Qom-Iran.
    3. Department of Mesenchymal Stem Cells, the Academic Center for Education, Culture and Research, Qom Branch, Qom-Iran.
    4. Department of Mesenchymal Stem Cells, the Academic Center for Education, Culture and Research, Qom Branch, Qom-Iran.
    5. Department of Mesenchymal Stem Cells, the Academic Center for Education, Culture and Research, Qom Branch, Qom-Iran.


  • Introduction: Because of its anti-neoplastic characteristics, the L-asparaginase enzyme is crucial to the chemotherapy process. Microbes are a useful source for extracting this enzyme even though it is found in several animals, microorganisms, and plants. The purpose of this study is to isolate and characterize the bacteria that produce the L-asparaginase enzyme in Hoz Sultan Lake.
  • Methods: Samples were cultivated in M9 specific culture medium to separate the enzyme-producing strains from the isolated bacteria. The enzyme activity of the strains was evaluated by colorimetric method. To identify microorganisms in the beginning, biochemical tests were used. With the aid of the PCR approach, the bacteria were further identified using 16S rRNA gene analysis. The sequences of the 3 robust strains, including Brevibacillus laterosporus strain, Brevibacillus laterosporus strain B9, and Paenibacillus dendritiformis strain S10-R2A-10—were immediately released publicly in the NCBI databases with the assigned accession numbers.
  • Results: This study indicates that the L-asparaginase enzyme may be produced by marine microorganisms. Brevibacillus laterosporus strain B9 is a very productive strain that can be used to make L-asparaginase.
  • Conclusion: In light of this findings, it is therefore reasonable to consider the Howz-Sultan Salt Lake as a potential bioresource for strains that generate L-asparaginase that could be advantageously used in future large-scale gene cloning for the food and medicinal industries.
  • Keywords: Halophilic Bacteria, L-asparaginase, Howz-Sultan Salt Lake, Isolation, Identification