Examining the pattern of bacterial isolates obtained from urinary infection in Imam Khomeini Hospital in Shirvan city in 2021
Examining the pattern of bacterial isolates obtained from urinary infection in Imam Khomeini Hospital in Shirvan city in 2021
Moein Hamidi hesari,1,*Simin Babazade,2
1. MSc in Microbiology, Imam Khomeini Hospital, Shirvan, North Khorasan University of Medical Sciences, Bojnourd, Iran 2. BA in Medical Laboratory Shirvan Imam Khomeini Hospital, Shirvan, North Khorasan University of Medical Sciences, Bojnourd, Iran
Introduction: Urinary tract infections are one of the major causes of complications and co-morbidities in patients with underlying diseases and constitute most of the reasons for going to the hospital worldwide. Sufficient knowledge of factors related to urinary tract infections can easily control the disease.Studies conducted in different communities show that gram-negative bacilli are the most common etiologic agents of urinary tract infections, and among them, Escherichia coli accounts for more than 80% of urinary tract infections. Considering the importance of urinary infections in this study, the prevalence rate and factors involved in causing urinary infections in Shirvan Imam Khomeini Hospital in 2021 were evaluated.
Methods: Urine samples were cultured in the laboratory of Imam Khomeini Hospital and the isolates isolated in positive cases were determined as uropathogens using microbiological and biochemical tests, including culture in blood agar media, mechanical and gram staining, test Citrate, urea test, bile squaline test, TSI environment, SIM test, 5.6% salt tolerance test, PYR test were performed.
Results: In 2021, a total of 5150 cases of urine culture were performed, of which 73 cases (4.7%) were positive. Among the positive cases, 65.7% were Escherichia coli, 20.55% Klebsiella species, 5.48% Pseudomonas aeruginosa, 2.73% Proteus mirabilis, 2.73% Streptococcus, 1.36% Staphylococcus saprophyticus and 1.36% Enterobacter.
Conclusion: According to the results, it is clear that the total number of positive cases of urine culture is reduced compared to previous studies, and among the positive cases, Escherichia coli is considered as the first cause of urinary infections by a large difference. The relatively low percentage of cases of Staphylococcus saprophyticus indicates a significant change in the causes of urinary infections from members of Gram-positive bacteria to Enterobacteriaceae.