The cytoprotective activity of an alcoholic extract of Conocarpus erectus against UVB rays in the skin cell line HSF-PI 17
The cytoprotective activity of an alcoholic extract of Conocarpus erectus against UVB rays in the skin cell line HSF-PI 17
Dian dayer,1,*Fatima farzam,2Parnian Fardaei,3Vahid Bayati,4
1. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 2. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 4. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Introduction: Given the beneficial effects of kenocarpus extract in healing skin wounds, this study looked into the impact of Conocarpus leaf extract in protecting HSF-PI 17 type fibroblast cells from the harmful effects of UVB rays.
Methods: Following cell culture, the MTT method was used to test the cytotoxicity of Conocarpus extract. The cells were divided into the control group, those receiving radiation only, and those receiving radiation and the Conocarpus section. Trypan blue staining was used to determine the number of viable cells. The amount of ROS produced was calculated by measuring the fluorescence colour intensity. Real-time PCR was used to assess gene expression, and Western blotting was used to determine protein expression.
Results: Conocarpus extract had no toxic effect on HSF-PI 17 cells in the doses used. The impact of radiation resulted in a significant increase in ROS production and a substantial decrease in the cell growth rate compared to the control group . In the third group, Conocarpus extract significantly moderated growth reduction and ROS production compared to the second group. TGF-β and SMAD2/3 gene expression and Collagen protein levels were significantly lower in the second group than in the control group (p<0.05). TGF-β and SMAD2/3 gene expression and collagen protein expression increased substantially in the third group compared to the second group.
Conclusion: Conocarpus leaf extract reduces the harmful effects of UVB rays in HSF-PI 17 skin fibroblast cells.