LAMA3 Overexpression as a Potential Contributor to Development of OC and LC and Its Predictive CeRNA Networks
LAMA3 Overexpression as a Potential Contributor to Development of OC and LC and Its Predictive CeRNA Networks
Reyhaneh Ghanbari,1Mohammad Rezaei,2Mansoureh Azadeh,3,*
1. Zist Fanavari Novin Biotechnology Institute 2. Zist Fanavari Novin Biotechnology Institute 3. Zist Fanavari Novin biotechnology institute
Introduction: Laryngeal cancer(LC) and oral cancer(OC) are types of head and neck squamous cell carcinoma(HNSC). HNSC usually begins in the squamous cells that line the mucosal surfaces of the head and neck(for example, inside the mouth and larynx). These cancers are known as squamous cell carcinoma of the head and neck. HNSC, usually diagnosed in elderly patients in association with heavy tobacco and alcohol use, is declining in part due to the decline in tobacco use worldwide. In this study, we focused on finding a common gene between LC and OC to finally find a CeRNA.
Methods: Initialy, OC dataset GSE19089 and LC dataset GSE143224 were obtained from NCBI Gene Expression Omnibus (GEO) and then analyzed by GEO2R; finally, the LAMA3 gene was selected for further work (Log FC > 1, adj. p value < 0.05).
Results: Based on microarray analysis, LAMA3 is significantly increased in patient samples(GEPIA2, ENCORI). Gene ontology and pathway analysis were performed by ENRICHR and KEGG databases to strengthen the relationship of this gene with OC and LC. Analysis of miRNA-mRNA interactions(miRWalk V.3) revealed hsa-miR-2681-5p to be a novel repressor for our gene(score = 1). We analyzed possible lncRNA-mRNA interactions using lncRRisearch and selected LINC01215, which was taken to GENECARDS for lncRNA verification. By analyzing lncRNA-miRNA interactions(lncBase V.3), we found that hsa-miR-2681-5p has a significant interaction with MALAT1 and NEAT1.
Conclusion: As a result, the LAMA3 gene may be a ceRNA along with MALAT1 and NEAT1. Also, the mentioned lncRNAs(MALAT1, NEAT1) may act as transcriptional regulators for numerous genes, including some involved in cancer metastasis and cell migration, and they are involved in cell cycle regulation.