Investigating ARRB1 gene expression under tumor microenvironment mimetic conditions in MDA-MB-231 cells
Investigating ARRB1 gene expression under tumor microenvironment mimetic conditions in MDA-MB-231 cells
Neda Khodapasand,1,*Rasoul Sharifi,2
1. Department Of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran. 2. Department Of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran.
Introduction: Cancer is a complex disease that can be defined as a
human disease with genetic heterogeneity. Because it is determined by a few special
adaptations, such as pH dysregulation is a major symptom in solid tumors.
Compared to normal tissue, solid tumors are characterized by extracellular
acidification and intracellular alkalinization. Therefore, the study focuses on the
different concentrations of glucose and hypoxia on the expression of the ARRB1
gene in the MDA-MB-231 breast cancer cell line.
Methods: The MDA-MB-231 cells were culture in RPMI culture
medium with 5.5, 11, and 25 mM glucose and oxygen of %1 and %21. In the next
step, the RNA was extracted and cDNA was synthesized. Finally, the expression of
ARRB1 mRNA is checked by real-time PCR.
Results: MDA-MB-231 cells in hypoxia conditions and over time compared to
other different treatments have adapted morphological. RAAB1 gene expression
showed a significant increase (P≤0.01) in 24 hours. While this increase in 48 hours
only in normal conditions of normoxia and 25 mM glucose at the level of P≤0.001 and
in 72 hours of expression at the level of P≤0.001 showed a decrease in expression.
Conclusion: Increasing or decreasing the concentration of glucose and
hypoxia changes the expression profile of cancer cells and can affect the condition
type and step.