Therapeutic effect of aerosol inhalation of exosomes from allogeneic fat mesenchymal stem cell culture in a pulmonary fibrosis model in rats
Therapeutic effect of aerosol inhalation of exosomes from allogeneic fat mesenchymal stem cell culture in a pulmonary fibrosis model in rats
fereshteh nejaddehbashi,1,*maryam radan,2atefeh ashtari,3vahid bayati,4dyan dayer,6
1. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2. Department of Physiology, Faculty of Medicine, Persian Gulf Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 3. Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 4. cellular and molecular research center, Medical Basic Sciences Institute,Ahvaz Jundishapur University of Medical Sciences,Ahvaz, Iran. Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 6. cellular and molecular research center, Medical Basic Sciences Institute,Ahvaz Jundishapur University of Medical Sciences,Ahvaz, Iran
Introduction: Pulmonary fibrosis is a chronic interstitial lung disease, which is caused by damage to the lung parenchyma by inflammatory factors and fibrosis. Today, research on extracellular vesicles derived from mesenchymal stem cells is one of the areas of interest in regenerative medicine. Studies show that therapeutic use of exosomes as a non-cellular treatment method has a number of advantages that can be a good justification for its replacement in conventional cell therapy methods.
Methods: In this study, we evaluate the effects of adipose-derived mesenchymal stem cells (adMSCs) originating exosomes to repair pulmonary fibrosis. Here we present a series of studies utilizing exosome by inhalation to treat models of lung injury and fibrosis. Male adult Sprague-Dawley (SD) rats were randomly divided into three groups: the control group, the exosome receiving group with a concentration of 500µg/ml, and 250 µg/ml. Tissue samples and the levels of oxidative stress and inflammatory factors in each group were compared.
Results: exosomes isolated ranged in size from 30 to 150 nm and demonstrated the characteristic cup-shaped morphology with TEM. We showed that an inhalation treatment of exosome exhibited therapeutic potential for lung regeneration in experimental models of pulmonary fibrosis. Pathologic alteration of lung tissue, levels of pro-inflammatory cytokines, were measured to evaluate the therapeutic effect of treatment with MSCs exosomes.
Conclusion: Our results demonstrate that exosomes, constitutively produced by adMSCs, have the potential to be utilised as a therapeutic tool for effective tissue-engineered lung.