Introduction: Kidney Renal Clear Cell Carcinoma (RCCs) is a glandular (adeno) carcinoma of the kidney, and it includes 85% of kidney cancers [1]. Importantly, RCC is a disease of increasing incidence. By using genomic data and genomic observations, an important step can be taken in the process of prevention and development of treatment methods [2].
The investigation aims to investigate gene expression SLC25A4 by measuring hsa-miR-370-3p at the cellular level of kidney Renal Clear Cell Carcinoma patients.
Methods: At the First, the gene expression data of RCC patients (GSE148795) was obtained from NCBI Gene Expression Omnibus (GEO), and then the expressed genes were searched by GEO2R and analyzed differently (GEPIA2) and (ENCORI). Then the Enrchr database was used to analyze the signaling pathway. Also, the complete form of the signaling pathway was obtained in the KEGG database. Through UniProt [3] and GeneCards [4], gene ontology information and biological pathway involvement were apprehended. In addition, miRWalk was used to find significant miRNA-mRNA interactions in the 3'UTR region. In addition, the selected miRNA was searched in LncBase v.3 [5] to find strong interactions with lncRNAs and complete a predictive ceRNA network.
Results: Through analysis of the GEO dataset, a gene named SLC25A4 was found to be considerably upregulated (logFC = -0.1913039, adj. P value =2.83E- 01) in RCC samples. The SLC25A4 gene provides the instructions for making a protein called adenine nucleotide translocase type 1 (ANT1). ANT1 functions in mitochondria, which are structures within cells that convert the energy from food into a form that cells can use [6]. Analysis of possible miRNA-mRNA interactions revealed hsa-miR-370-3p as a significant interactor to SLC25A4 mRNA. This miRNA was then searched in LncBase v.3, and AD000090.1, KCNQ1OT1, NAMPTP1, and SAR1AP1 had the strongest interactions.
Conclusion: Thus, SLC25A4 is overexpressed in RCC and Forms a possible CeRNA network among hsa-miR-370-3p, and AD000090.1, KCNQ1OT1, NAMPTP1 and SAR1AP1.