KRAS and GSK Critical Kinases Biomarkers in Astrocyte and Oligodendrocyte Derived Amyotrophic Lateral Sclerosis
KRAS and GSK Critical Kinases Biomarkers in Astrocyte and Oligodendrocyte Derived Amyotrophic Lateral Sclerosis
Ehsan Asghari Jafari,1Maryam Arabi,2Ahmad Bereimipour,3,*
1. Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Medical Genomics Research Center, Tehran Medical Sciences Islamic Azad University, Tehran, Iran 3. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Introduction: Amyotrophic Lateral Sclerosis (ALS), a degenerative illness that can lead to death, begins with the loss of motor neurons in the brain and spinal cord. Medical researchers are still trying to figure out molecular mechanisms in neurological diseases like ALS. Both astrocytes and oligodendrocyte dysfunction can hasten the progression of the disease in this case.
Methods: A bioinformatics approach was used to look at the molecular mechanisms and discover important elements between these two cell types in ALS. In this study, we looked at genes, protein products, and miRNAs in astrocytes and oligodendrocytes utilizing integrated and continuous bioinformatics analytics via multiple tools and databases.
Results: Cellular senescence, actin cytoskeleton, and cell cycle signaling pathways were all involved in the findings acquired. When all the information was analyzed, TP53, MDM2, KRAS, PTPRC, and GSK proteins were identified as possible targets of hsa-miR-496-5p, hsa-miR-396-5p, and hsa-miR-4258-3p miRNAs, respectively.
Conclusion: Finally, in this investigation of ALS produced from astrocytes and oligodendrocytes, the four genes had a more robust and better association.