Introduction: Pancreatic cancer (PAAD) is malignancies which is predominantly seen in men and at advanced age (40-85) and has an aggressive course. It accounts for2% of all cancers and 5% of cancer-related deaths .the disease is very difficult to detect as it has no early signs and spreads rapidly to surrounding organs is one of most deadly types of cancer.(1)
Methods: This investigation aimed to study expressed gene(VCAN)in data of pancreatic ductal adenocarcinoma and normal tissue . Expression analysis of GSE132956 achieved from GEO2R online software and validation of expression analysis performed by GEPIA2 and ENCORI database.(2)Single nucleotide polymorphisms (SNPs) of VCAN extracted from d b SNP and identification of deleterious SNPs Brought out from SIFT database (3)Based on microarray analysis ,VCAN have significant up-regulation in the PAAD samples,(fold change,.27_p.value0.013).(4)database was then used to reinforce the possibility of correlation between the VCAN gene and PPAD cancer. furthermore , mi R Walk were utilized to find significant mi RNA-mRNA interaction in the 3UTR region .(5)
Results: Analysis of possible mi RNA –mRNA interaction revealed has-miR-6895-3p as a significant connection to VCAN mRNA .This mi RNA was a novel mi RNA in the Pub med database .(6)The VCAN gene have 8number Ln c RNA in the Ln c R research data base and were RNA gene and significant Ln c RNA in the gene card data base .(7)The mi RNA have expression and survival significant by PAAD samples in the data base ENCORY .
Conclusion: In conclusion ,VCAN gene is a high expressed in PPAD patient and have assoieate significant by miRNA (has –miR -6895-3P)and ENST00000627551 and 7 number Lnc RNA another that to create a network ce RNA,